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ChemiScreen™ M1 Calcium-Optimized FLIPR Cell Lines
Description:
ChemiScreen™ M1 Calcium-Optimized FLIPR Cell Lines
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
2 vials
Background Information:
The muscarinic acetylcholine receptor family consists of five GPCRs that mediate some
of the neurotransmission functions of acetylcholine in the CNS and the periphery. The
M1 receptor, along with the M3 and M5 receptors, signal through Gq/11 and subsequent
release of Ca++ from the ER. The M1 receptor is expressed in ganglia and mediates
depolarization of ganglia by inhibition of voltage-gated M-type K+ channels. In addition,
the M1 receptor mediates venous contraction (Caulfield and Birdsall, 1998). Chemicon’s
cloned human M1-expressing cell line is made in the Chem-1 host, which supports high
levels of recombinant M1 expression on the cell surface and contains high levels of the
promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.
Thus, the cell line is an ideal tool for screening for antagonists of interactions between M1
and its ligands.
of the neurotransmission functions of acetylcholine in the CNS and the periphery. The
M1 receptor, along with the M3 and M5 receptors, signal through Gq/11 and subsequent
release of Ca++ from the ER. The M1 receptor is expressed in ganglia and mediates
depolarization of ganglia by inhibition of voltage-gated M-type K+ channels. In addition,
the M1 receptor mediates venous contraction (Caulfield and Birdsall, 1998). Chemicon’s
cloned human M1-expressing cell line is made in the Chem-1 host, which supports high
levels of recombinant M1 expression on the cell surface and contains high levels of the
promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.
Thus, the cell line is an ideal tool for screening for antagonists of interactions between M1
and its ligands.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.
UniProt Summary:
FUNCTION: SwissProt: P11229 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
SIZE: 460 amino acids; 51421 Da
SUBUNIT: Interacts with GPRASP2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P11229 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 460 amino acids; 51421 Da
SUBUNIT: Interacts with GPRASP2.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P11229 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Cell Type:
Chem-1
Cell Line Type:
GPCR Cell Lines
Brand Family:
Chemicon
Host Cells:
Chem-1
Protein Target:
M1
Format:
Cell Line
UniProt Number:
Ligand Type:
non-peptide
Target Sub-Family:
Acetylcholine (muscarinic)
Promotional Text:
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GPCR Class:
A
Packaging:
1 mL per vial
Gene Symbol:
- CHRM1
- MGC30125
- M1
- HM1
Protein or Enzyme Type:
GPCRs
Product Name:
ChemiScreen™ M1 Calcium-Optimized FLIPR Cell Lines
Entrez Gene Number: