Content Loading...
Content Loading...
Product Images
Figure 1. Binding of [35S]-GTPγS to CXCR6 membrane preparation. Two lots of CXCR6 Membrane Preparation (catalog # HTS054M) and Wild-type ...
Figure 2. Competition radioligand binding for CXCR6. 10 μg/well CXCR6 Membrane Preparation (HTS054M) and Wild-Type Chem-1 Membrane Preparation (HTS...
ChemiScreen™ CXCR6 Membrane Preparation
Description:
ChemiScreen™ CXCR6 Membrane Preparation
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
200 units
Product Overview:
Human CXCR6 cDNA
Background Information:
The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001) CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005). CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Chemicon's CXCR6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR6 interactions with its ligands. The membrane preparations exhibit a Kd of 0.6 nM for [125I]-CXCL16. With 2.5 or 10 μg/well CXCR6 Membrane Prep and 0.5 nM [125I]-CXCL16, greater than 4-fold and 9-fold signal-to-background ratios are obtained, respectively.
Application Notes:
Radioligand binding assay, and GTPγS binding.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: O00574 # Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.
SIZE: 342 amino acids; 39280 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in lymphoid tissues and activated T cells.
SIMILARITY: SwissProt: O00574 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 342 amino acids; 39280 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in lymphoid tissues and activated T cells.
SIMILARITY: SwissProt: O00574 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Quality Assurance:
SPECIFICATIONS:
1 unit = 5 μg for GTPγS binding assay
EC50 in GTPγS binding assay by CXCL16: 0.52 – 1.2 nM
1 unit = 5 μg for GTPγS binding assay
EC50 in GTPγS binding assay by CXCL16: 0.52 – 1.2 nM
Cell Type:
Chem-1
Brand Family:
Chemicon
Incubation Conditions:
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] CXCL16 (Perkin Elmer catalog # NEX398)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled CXCL16 at 0.5 nM
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] CXCL16 (Perkin Elmer catalog # NEX398)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled CXCL16 at 0.5 nM
Host Cells:
Chem-5
Presentation:
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL16-stimulated [35S]-GTPγS binding. If radioligand binding assays are desired, it is recommended to double the mass of membrane prep per well to increase signal window..
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Protein Target:
CXCR6
Format:
Membranes
UniProt Number:
Ligand Type:
peptide
Target Sub-Family:
Chemokine
Storage Conditions:
Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
Promotional Text:
For special offers Click here http://www.millipore.com/drugdiscovery/dd3/gpcrtargetsolutions
GPCR Class:
A
Gene Symbol:
- CXCR6
- CDw186
- BONZO
- CXC-R6
- CXCR-6
- TYMSTR
- CD186
- STRL33
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiScreen™ CXCR6 Membrane Preparation
Entrez Gene Number:
Alternate Names:
- BONZO
- STRL33
- TYMSTR
Cell Membrane Type:
GPCR