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  • : HTS109C
  • : 2 vials
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Calcium flux in 5-HT2B-expressing CHO cell line induced by α-methyl- 5-hydroxytryptamine. 5-HT2B-expressing CHO cells and Wild-...

ChemiScreen™ Human 5-HT2B Serotonin Receptor Calcium-Optimized FLIPR Cell Line

Description:
ChemiScreen™ Human 5-HT2B Serotonin Receptor Calcium-Optimized FLIPR Cell Line
Trade Name:
  • ChemiScreen
  • Chemicon (Millipore)
Qty/Pk:
2 vials
Product Overview:
Full-length human HTR2B cDNA encoding 5-HT2B
Background Information:
5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al., 1994). The 5-HT2B receptor, which couples to Gq/11 to increase intracellular calcium, is expressed in embryonic and adult cardiovascular tissues, gut and brain from the rat, mouse, and human species. A role for 5-HT2B receptors was suggested in the cardiopathy associated with fenfluramine (Fitzgerald et al., 2000). Chemicon's cloned human 5-HT2B -expressing cell line is made in the CHO host, which supports high levels of recombinant 5-HT2B expression on the cell surface. Thus, the cell line is an ideal tool for screening for antagonists of interactions between 5-HT2B and its ligands.
Applications:
Human 5-HT2B GPCR stably expressed in CHO cells for Calcium flux FLIPR Assays.
Application Notes:
Calcium flux assay, ligand binding assays
Species Reactivity:
Human
Usage Statement:
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Multiple receptor subtypes of serotonin neurotransmitters with multiple physiologic functions have been recognized. The 5-HT-2 receptors mediate many of the central and peripheral physiologic functions of serotonin. Cardiovascular effects include contraction of blood vessels and shape changes in platelets; central nervous system effects include neuronal sensitization to tactile stimuli and mediation of hallucinogenic effects of phenylisopropylamine hallucinogens.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P41595 # This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 481 amino acids; 54298 Da
SUBUNIT: Interacts with MPDZ.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Detected in most peripheral organs. Only low expression levels were found in the brain.
SIMILARITY: SwissProt: P41595 ## Belongs to the G-protein coupled receptor 1 family.
Species:
Human
Brand Family:
Chemicon
Presentation:
Cells are frozen at 2 x 106 cells/mL in F-12K Nutrient Mixture, Kaighn's Modification /20% dialyzed heat inactivated fetal bovine serum /10% DMSO. Cell line tests negative for mycoplasma.
Protein Target:
5-HT2B
Storage Conditions:
Place cells in liquid nitrogen immediately upon receipt. Maintain frozen in liquid nitrogen for up to 5 years. Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37ºC water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol. Transfer contents of the vial to a T75 flask containing 20 mL growth media, and place in a humidified 37°C incubator with 5% CO2.. After 8-24 h, cells will adhere to the plate, at which time the media should be replaced to remove residual DMSO. Cells are passaged by washing with Ca++ and Mg++-free HBSS (10 mL/T75), incubating with 0.05% trypsin/0.2 g/L EDTA (1 mL/T75) for 5-10 minutes at 37°C, and rapping the side of the flask to dislodge the cells. Neutralize the trypsin by addition of 4 volumes growth media. Cells are typically passaged 1:10 with every 3-4 days, and should be passaged at least once after thawing prior to use in calcium flux assays.
Target Sub-Family:
Serotonin
GPCR Class:
Promotional Text:
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Gene Symbol:
  • HTR2B
  • 5-HT-2B
  • 5-HT2B
  • 5-HT(2B)
Product Name:
ChemiScreen™ Human 5-HT2B Serotonin Receptor Calcium-Optimized FLIPR Cell Line
Materials Required but Not Delivered:
F-12K Nutrient Mixture, Kaighn's Modification /10% dialyzed heat inactivated fetal bovine serum/0.25 mg/mL Geneticin (G418)/100 U/mL each penicillin and streptomycin.
Quality Assurance:
EC50 for calcium mobilization by α-methyl-5-hydroxytryptamine: ~ 0.35nM
Signal:noise at agonist Emax: 244
Host Cells:
CHO (Chinese hamster ovary cells)
UniProt Number:
Packaging:
1 mL per vial, 2 x 106 cells/mL
Entrez Gene Number:

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