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Figure 1. Binding of [35S]-GTPγS to MrgX1 membrane preparation. 5 µg/well MrgX1 Membrane Preparation (catalog # HTS122M) and Wild-type Chem-1 Membra...
ChemiSCREEN™ Membrane Preparation Recombinant Human MrgX1 Receptor
Description:
ChemiSCREEN™ Membrane Preparation Recombinant Human MrgX1 Receptor
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
200 units
Product Overview:
Human MrgX1
Background Information:
The Mas-related gene family (Mrg), also known as SNSR (sensory neuron-specific G protein-coupled receptors), are expressed mainly in the dorsal root ganglia and appear to be involved in nociception and other sensory processes. MrgX1/SNSR4 is activated by the neuropeptide BAM22, and it mediates neuronal synaptic transmission and ion channel activity (Lembo et al., 2002; Burstein et al., 2006, Chen and Ikeda, 2004). Millipore's MrgX1 Membrane Preparation for GTPγS Assay is a crude membrane preparation made from the proprietary stable recombinant cell line that is also used for Millipore’s MrgX1 Calcium Optimized Cell Line (HTS122C). This MrgX1 Membrane Preparation has been optimized for low basal activity in GTPγS binding assays; thus it is an ideal HTS tool for screening of compounds for agonist and antagonist activities. The membrane preparations exhibits EC50 of 93.7 nM for BAM(8-22) in a GTPγS binding assay.
Application Notes:
Radioligand binding assay and GTPγS binding
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
1 unit = 5 μg
EC50 in GTPγS binding assay by BAM (8-22): ~ 93.7 nM
EC50 in GTPγS binding assay by BAM (8-22): ~ 93.7 nM
Cell Type:
Chem-1
Brand Family:
Chemicon
Incubation Conditions:
Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled BAM (8-22) was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific BAM(8-22)-stimulated [35S]-GTPγS binding.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific BAM(8-22)-stimulated [35S]-GTPγS binding.
Host Cells:
Chem-1
Presentation:
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Protein Target:
MRGX1 / MRGPRX1
Ligand Type:
peptide
Storage Conditions:
Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
Target Sub-Family:
Mas-related gene
Promotional Text:
For special offers Click here http://www.millipore.com/drugdiscovery/dd3/gpcrtargetsolutions
GPCR Class:
A
Protein or Enzyme Type:
GPCR Membrane Preparations
Product Name:
ChemiSCREEN™ Membrane Preparation Recombinant Human MrgX1 Receptor
Entrez Gene Number:
Cell Membrane Type:
GPCR