READY-TO-ASSAY™ CALCIUM-OPTIMIZED CELLS
HUMAN RECOMBINANT GIP GLUCAGON FAMILY RECEPTOR
Description:
READY-TO-ASSAY™ CALCIUM-OPTIMIZED CELLS
HUMAN RECOMBINANT GIP GLUCAGON FAMILY RECEPTOR
HUMAN RECOMBINANT GIP GLUCAGON FAMILY RECEPTOR
Trade Name:
Chemicon (Millipore)
Product Overview:
Full-length human GIPR cDNA encoding GIP receptor
Background Information:
Millipore’s Ready-To-Assay™ Calcium-Optimized Cells are GPCR-expressing cell lines that are designed for simple, rapid calcium assays with no requirement for culturing cells. The user simply thaws the cells with maximal viability, dispenses into assay plates, and assays for calcium response the next day.
The Ready-To-Assay™ cells are derived from ChemiScreen™ calcium-optimized stable cell lines, which express the GPCR target of interest at high levels on the cell surface, in a host cell line containing high levels of the promiscuous Gα15 protein to couple the receptor to the calcium signaling pathway. The Ready-To-Assay™ cells are prepared by chemical treatment at a concentration optimized for effective growth arrest while maintaining high viability (>80%) after thawing and overnight plating. Pharmacological functionality of the Ready-To-Assay™ cells is identical to that of the originating GPCR cell line.
Gastric inhibitory polypeptide receptor (GIP) has been identified in the glucose-mediated secretion of insulin(Mayo et al., 2003). GIP is in the secretin/VIP receptor family which includes secretin, VIP, glucagon, GLP-1, growth hormone releasing hormone (GHRH), and PACAP (Yip et al., 1999). GIP is secreted after meal ingestion has been shown to stimulate bone formation resulting in lower occurrences of osteoporosis (Tsukiyama et al., 2006). Type 2 diabetes is a result of decreased glucose-stimulated insulin secretion which makes insulin secretion potentiators a popular target for diabetes treatments; a defect in GIP expression and/or signaling may lead to β-cell dysfunction and type 2 diabetes (Mayo et al., 2003). Millipore’s cloned human GIP receptor-expressing cell line is made in the Chem-9 host, an adherent cell line that supports high levels of recombinant GIP receptor expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. The untreated GIP receptor-Chem-9 cell line and the Ready-To-Assay™ GIP receptor cells have equivalent EC50s for GIP.
The Ready-To-Assay™ cells are derived from ChemiScreen™ calcium-optimized stable cell lines, which express the GPCR target of interest at high levels on the cell surface, in a host cell line containing high levels of the promiscuous Gα15 protein to couple the receptor to the calcium signaling pathway. The Ready-To-Assay™ cells are prepared by chemical treatment at a concentration optimized for effective growth arrest while maintaining high viability (>80%) after thawing and overnight plating. Pharmacological functionality of the Ready-To-Assay™ cells is identical to that of the originating GPCR cell line.
Gastric inhibitory polypeptide receptor (GIP) has been identified in the glucose-mediated secretion of insulin(Mayo et al., 2003). GIP is in the secretin/VIP receptor family which includes secretin, VIP, glucagon, GLP-1, growth hormone releasing hormone (GHRH), and PACAP (Yip et al., 1999). GIP is secreted after meal ingestion has been shown to stimulate bone formation resulting in lower occurrences of osteoporosis (Tsukiyama et al., 2006). Type 2 diabetes is a result of decreased glucose-stimulated insulin secretion which makes insulin secretion potentiators a popular target for diabetes treatments; a defect in GIP expression and/or signaling may lead to β-cell dysfunction and type 2 diabetes (Mayo et al., 2003). Millipore’s cloned human GIP receptor-expressing cell line is made in the Chem-9 host, an adherent cell line that supports high levels of recombinant GIP receptor expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. The untreated GIP receptor-Chem-9 cell line and the Ready-To-Assay™ GIP receptor cells have equivalent EC50s for GIP.
Application Notes:
Calcium flux assay
Species Reactivity:
Human
Host Cells:
Chem-9, an adherent cell line expressing the promiscuous G-protein.
Packaging:
1 x 107 viable cells/mL
Materials Required but Not Delivered:
PLATING MEDIA:
DMEM with 4.5 g/L glucose and 4 mM glutamine (Millipore SLM-020-A)
10% heat-inactivated FBS
1x Nonessential amino acids (from 100x stock, Millipore TMS-001-C)
10mM HEPES (from 1 M HEPES, Millipore TMS-003-C)
100 U/mL Pen-Strep (from 100x stock, Millipore TMS-AB2-C)
DMEM with 4.5 g/L glucose and 4 mM glutamine (Millipore SLM-020-A)
10% heat-inactivated FBS
1x Nonessential amino acids (from 100x stock, Millipore TMS-001-C)
10mM HEPES (from 1 M HEPES, Millipore TMS-003-C)
100 U/mL Pen-Strep (from 100x stock, Millipore TMS-AB2-C)
Gene Symbol:
- GIPR
- MGC126722
- GIP-R
Analytes Available:
GIP
Presentation:
Cells are frozen at 1 x 107 cells/mL in DMEM/20% fetal bovine serum/100 U/ml penicillin and streptomycin/10% DMSO.
Quality Assurance:
| EC50 for GIP (nM) | Maximum Signal (RFU) | Z' | |
| Ready-To-Assay Cells | 5.4 | 2202 | 0.73 |
| Continuous Passage Cells | 5.7 | 3138 | 0.72 |
Detection Methods:
Radioactive
Kit or Assay Type:
Cell Based Assays
Protein or Enzyme Type:
GPCRs
Species:
Human
Storage Conditions:
Place cells in liquid nitrogen immediately upon receipt. Maintain frozen in liquid nitrogen for up to 5 years.
1) Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37ºC water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol.
2) Transfer contents of the vial to a sterile 15 mL conical tube. Add 10 mL prewarmed plating media to the cells and mix gently to resuspend cells. Centrifuge at 200 x g. Remove all but 0.5 mL media.
3) Resuspend cells to 0.5 x 106 cells/mL in plating media. Dispense the cell suspension into a 96-well assay plate at 200 µL per well to obtain a density of approximately 1 x 105 cells/well.
4) Place the assay plate in a humidified 37°C incubator with 5% CO2.
5) The cells may be assayed 16-24 hours after plating. It is recommended to wash the cells with assay buffer at least once prior to addition of loading dye.
1) Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37ºC water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol.
2) Transfer contents of the vial to a sterile 15 mL conical tube. Add 10 mL prewarmed plating media to the cells and mix gently to resuspend cells. Centrifuge at 200 x g. Remove all but 0.5 mL media.
3) Resuspend cells to 0.5 x 106 cells/mL in plating media. Dispense the cell suspension into a 96-well assay plate at 200 µL per well to obtain a density of approximately 1 x 105 cells/well.
4) Place the assay plate in a humidified 37°C incubator with 5% CO2.
5) The cells may be assayed 16-24 hours after plating. It is recommended to wash the cells with assay buffer at least once prior to addition of loading dye.