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Calcium flux in GPR41-expressing Chem-1 cell line induced by Sodium Propionate. GPR41-expressing Chem-4 cells and Wild-Type Chem-4 cells (Chemicon ca ...
ChemiScreen™ Human Recombinant GPR41 Free Fatty Acid Receptor Calcium-Optimized Stable Cell Line
Description:
ChemiScreen™ Human Recombinant GPR41 Free Fatty Acid Receptor Calcium-Optimized Stable Cell Line
Trade Name:
ChemiScreen
Background Information:
GPR41 is a GPCR that, along with GPR43, is activated by short chain carboxylic acids formate, acetate, proprionate, butyrate and pentanoate (Brown et al., 2003; Brown et al., 2005). Binding of these ligands to GPR41 selectively activates Gi to inhibit cAMP accumulation. Expression of GPR41 is prominent in adipose tissue, increases during differentiation of cultured adipocytes, and allows short chain carboxylic acids to stimulate leptin synthesis in adipocytes (Xiong et al., 2003). Chemicon's cloned human GPR41-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant GPR41 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between GPR41 and its ligands.
Application Notes:
Calcium flux assay, ligand binding assays
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Line Type:
GPCR Cell Lines
Host Cells:
Chem-4, an adherent cell line expressing the promiscuous G-protein, Gα15.
Packaging:
2 x 106 cells/vial
Cell Type:
Chem-4
Gene Symbol:
- FFAR3
- GPR41
- FFA3R
Presentation:
Cells are frozen at 2 x 106 cells/mL in DMEM/20% fetal bovine serum/100 U/ml penicillin and streptomycin/10% DMSO. Cell line tests negative for mycoplasma.
Quality Assurance:
EC50 for calcium mobilization by Sodium Propionate: ~ 16 uM
TRANSFECTION: Full-length human GPR41 cDNA
GROWTH MEDIA: DMEM containing 4.5 g/L glucose/10% heat inactivated fetal bovine serum/1x nonessential amino acids/10 mM HEPES/0.25 mg/ml Hygromycin (500 ug/ml) Geneticin (G418)/100 U/ml each penicillin and streptomycin.
TRANSFECTION: Full-length human GPR41 cDNA
GROWTH MEDIA: DMEM containing 4.5 g/L glucose/10% heat inactivated fetal bovine serum/1x nonessential amino acids/10 mM HEPES/0.25 mg/ml Hygromycin (500 ug/ml) Geneticin (G418)/100 U/ml each penicillin and streptomycin.
Protein or Enzyme Type:
GPCRs
Species:
Human
Storage Conditions:
Place cells in liquid nitrogen immediately upon receipt. Maintain frozen in liquid nitrogen for up to 5 years. Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37°C water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol. Transfer contents of the vial to a T75 flask containing 20 mL growth media, and place in a humidified 37°C incubator with 5% CO2. After 8-24 h, cells will adhere to the plate, at which time the media should be replaced to remove residual DMSO. Cells are passaged by washing with Ca++ and Mg++-free HBSS (10 mL/T75), incubating with 0.05% trypsin/0.2 g/L EDTA (1 mL/T75) for 5-10 minutes at 37°C, and rapping the side of the flask to dislodge the cells. Neutralize the trypsin by addition of 4 volumes growth media. Cells are typically passaged 1:10 with every 3-4 days, and should be passaged at least once after thawing prior to use in calcium flux assays.