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Chem-3 cells were seeded in RPMI-1640/10%FBS/100U/ml Penicillin/ Streptomycin without supplement or with 1:100 Chem-3 Growth Supplement at 5x104<...
ChemiScreen™ Chem-3 Parental Cell Line
Description:
ChemiScreen™ Chem-3 Parental Cell Line
Trade Name:
- ChemiScreen
- Chemicon (Millipore)
Qty/Pk:
2 vials
Background Information:
Chemicon's Wild-Type Chem-3 cell line is a control for Chemicon's GPCR-expressing Chem-3 calcium-optimized cell lines. The Chem-3 cell line grows in suspension and contains high levels of the promiscuous G protein Galpha15 to couple many classes of GPCRs to the calcium signaling pathway.
Application Notes:
Negative control for calcium flux assay, ligand binding assays
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Mouse
Cell Type:
Chem-3
Cell Line Type:
GPCR Cell Lines
Brand Family:
Chemicon
Host Cells:
Chem-3
Presentation:
Cells are frozen at 2 x 10e6 cells/mL in RPMI/20% fetal bovine serum containing 10% DMSO. Cell line tests negative for mycoplasma.
Storage Conditions:
Place cells in liquid nitrogen immediately upon receipt. Maintain frozen in liquid nitrogen for up to 5 years. Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37ºC water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol. Transfer contents of the vial to a sterile conical tube containing 10 mL Growth Media, and centrifuge at 200 x g. Remove supernatant and resuspend in fresh Growth Media. Transfer to a T75 flask containing 20 mL final volume growth media, and place in a humidified 37°C incubator with 5% CO2. Cells should be maintained between 0.1 x 10e6 and 1.0 x 106 cells/mL, and are typically passaged 1:10 every 3-4 days. Cells should be passaged at least once after thawing prior to use in calcium flux assays
Target Sub-Family:
Control
Promotional Text:
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Packaging:
1 mL per vial
Protein or Enzyme Type:
GPCRs
Product Name:
ChemiScreen™ Chem-3 Parental Cell Line
Materials Required but Not Delivered:
GROWTH MEDIA: RPMI-1640/10% heat inactivated fetal bovine serum/100 U/ml each penicillin and streptomycin containing 1:100 dilution of Chem-3 Growth Supplement (Chemicon catalog # HTSCHEM-3S)
Concentration:
2 x 10e6 cells/mL