Ordering Information

Catalogue Number : IL038
Qty/Pk : 10 µg

Interleukin-1β, recombinant human

Description:

Trade Name:

Chemicon (Millipore)

Qty/Pk:

10 µg

Product Overview:

Interleukin-1beta (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T-cells. Human IL-1beta is a 17.3 kDa protein containing 153 amino acid residues.

Key Applications:

Cell Culture

Application Notes:

For most in vitro applications, human IL-1beta is fully biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Leukocytes.

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

UniProt Summary:

FUNCTION: SwissProt: P01584 # Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
SIZE: 269 amino acids; 30748 Da
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
DOMAIN: SwissProt: P01584 The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
SIMILARITY: Belongs to the IL-1 family.

Species:

Human

Stem Cell Type:

Hematopoietic Stem Cells

Stem Cell Workflow Stage:

Maintenance & Expansion
Differentiation
General Cell Culture

Brand Family:

Chemicon

Presentation:

Sterile filtered and lyophilized from 10 mM Tris, pH 8.2 with no preservatives.

UniProt Number:

P01584

Specific Activity:

Chemicon's human IL-1beta is fully biologically active when compared to standards. The ED(50) as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is < 0.001 ng/mL corresponding to a specific activity of > 1 x 10e9 units/mg.

Storage Conditions:

The lyophilized human IL-1beta, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted human IL-1beta should be stored in undiluted aliquots at -20°C for up to six months.

Cell Culture Reagent Type:

Cytokines & Growth Factors

Gene Symbol: