Leukemia Inhibitory Factor, recombinant rat

Leukemia Inhibitory Factor, recombinant rat

Chemicon (Millipore)

5 µg

Rat LIF (rtLIF) is a 22.1 kDa protein containing 202 amino acid residues that exhibits 91% amino acid sequence identity with murine LIF (Takahama etal 1998). Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama, et al 1998).

Manufactured by CHEMICON International, Inc. Rat LIF is not available for resale.

For research use only; not for use as a diagnostic.

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

• Stem Cell Culture
• Cell Culture

RECOMMENDED QC PROTOCOL

M1 Bioassay

1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3.Add 100 μL of 5% FCS in isotonic saline to two control slides.

4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).

Visit www.esgro-lif.com for additional information

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.

FUNCTION: SwissProt: P17777 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22113 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P17777 ## Belongs to the LIF/OSM family.

Rat

• Mouse Embryonic Stem Cells
• Neural Stem Cells
• Hematopoietic Stem Cells
• Mesenchymal Stem Cells
• Induced Pluripotent Stem Cells

• Maintenance & Expansion
• Differentiation
• General Cell Culture

Chemicon

rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

Liquid in 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl, pH 7.4 and 0.02% Tween 20. No preservativies added.

P17777

Rat LIF is shipped on dry ice. Maintain at -20°C until expiration date. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added and stored at -20°C. Freeze thawing is not recommended.

The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(e8) units/mg, where 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

Cytokines & Growth Factors

NM_022196

Cytokines & Growth Factors

Leukemia Inhibitory Factor, recombinant rat

LIF

10 μg/mL

Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests.