MultiScreen plates are available with a variety of specialty membranes for different applications. Positively or negatively charged membranes, nylon mesh and Ultrafiltration membrane products are some examples of what is offered.
Montage SEQ kits provide efficient, centrifugation-free sequencing reaction cleanup in 96 and 384-well formats. The unique size-exclusion separation technology eliminates the need for costly gel filtration resins or bind-wash-elute columns. In addition, the kits are optimized for use with BigDye v 3.0 and DYEnamic ET Terminator sequencing chemistries.
Developed to meet the needs of our customers, MultiScreen plates are proven as optimized, reliable tools for the advancement of drug discovery and life science research. We are committed to providing a premium quality product coupled with a high level of technical support.
ABI supplies a 5X buffer with BigDye version 3.1 and 1.1 chemistries. Can I use that buffer with the Montage SEQ96 kit? With Montage SEQ384 plates?
» Answer
You can use the ABI 5X buffer for dilution of sequencing reactions following the ABI protocol. This 5X buffer is equivalent to Millipore's 2.5X buffer, so you no longer need to make up a separate buffer for sequencing reaction dilutions. If you are using a BigDye chemistry that does not include ABI's buffer (versions 1.0, 2.0, 3.0) you have the option to purchase the ABI buffer and using it per ABI's recommendations or continuing to use Millipore's 2.5X buffer as before.