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Ordering Information
- : MAB079-1
- : 100 µL
- : Variable
Anti-S-100 Protein, clone 15E2E2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, B, M, R, Ca, Rb, Fe | IH, IH(P), IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-S-100 Protein, clone 15E2E2
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes intact, alpha and beta subunits of bovine, human, and murine S-100 protein, as determined by immunoprecipitation profiles.
Epitope:
Recognizes both alpha & beta S100
Immunogen:
Purified bovine S-100 protein
Clone:
15E2E2
Isotype:
IgG2aκ
Background Information:
S-100 protein is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. The protein exists in both heterodimeric and homodimeric forms. The two subunits of S-100 protein are the products of separate genes that are differentially expressed by various cells. The beta subunit is present in all S-100 positive cells and tumors. In contrast, the alpha subunit is detectable only in heart, skeletal muscle and brain. In addition to its calcium binding properties, S-100 protein has a high-affinity binding site for zinc and is involved in the regulation of protein phosphorylation in the brain. The protein apparently plays a role in cell differentiation and growth, cytoskeletal structure and function, and has been implicated in neuropathological diseases.
Species Reactivity:
- Human
- Bovine
- Mouse
- Rat
- Canine
- Rabbit
- Feline
Species Reactivity Note:
Bovine, human, mouse, and rat.
Application Notes:
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IP.
Visualization of cellular and subcellular localization of S-100 protein using immunohistochemical techniques.
Immunohistochemistry:
A 1:100-200 dilution of a previous lot was used for paraffin embedded tissue sections.
Optimal working dilutions must be determined by end user.
Control:
Positive Control Tissue: Melanoma
Quality Assurance:
Evaluated by Western Blot on PC12 lysates
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Western Blot Analysis:
1:1000 dilution of this antibody detected S100 on 10 µg of PC12 lysate.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG2aκ in buffer containing PBS, pH 7.6, 1% BSA and 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- S100A1
- S100A
- S100
- S100-alpha
Alternate Names:
Calcium Binding Protein A1/B1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
SIZE: 94 amino acids; 10546 Da
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.
SIZE: 94 amino acids; 10546 Da
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Packaging:
@ 1 mg/mL concentation
Product Name:
Anti-S-100 Protein, clone 15E2E2
Concentration:
Variable
Antibody Type:
Monoclonal Antibody