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Western Blot Analysis:
Representative lot data.
Human placenta lysates were resolved by electrophoresis, transferred to PVDF and probed with...
Anti-TLR8, clone 3H2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-TLR8, clone 3H2
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody recognizes TLR8.
Molecular Weight:
120kDa
Immunogen:
Recombinant human TLR8
Clone:
3H2
Isotype:
IgG2bκ
Background Information:
Toll like receptor 8 (TLR8) family in mammals is comprised of a family of transmembrane proteins which contain multiple leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domains. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa.
Species Reactivity:
Human
Species Reactivity Note:
Human
Control:
Human placenta lysate
Quality Assurance:
Evaluated by western blot on human placenta lysate.
Western Blot Analysis: 1-2 µg/mL of this antibody detected TLR8 in human placenta tissue lysate.
Western Blot Analysis: 1-2 µg/mL of this antibody detected TLR8 in human placenta tissue lysate.
Purification Method:
Protein G purfied
Presentation:
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, and 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Gene Symbol:
- TLR8
- MGC119600
- ORFNames=UNQ249/PRO286
- MGC119599
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q9NR97 # Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).
SIZE: 1041 amino acids; 119828 Da
SUBUNIT: Binds MYD88 via their respective TIR domains (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (By similarity).
TISSUE SPECIFICITY: Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
SIMILARITY: SwissProt: Q9NR97 ## Belongs to the Toll-like receptor family. & Contains 24 LRR (leucine-rich) repeats. & Contains 1 TIR domain.
SIZE: 1041 amino acids; 119828 Da
SUBUNIT: Binds MYD88 via their respective TIR domains (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (By similarity).
TISSUE SPECIFICITY: Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.
SIMILARITY: SwissProt: Q9NR97 ## Belongs to the Toll-like receptor family. & Contains 24 LRR (leucine-rich) repeats. & Contains 1 TIR domain.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-TLR8, clone 3H2
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody