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Ordering Information
- : MAB1049
- : 100 μg
- : 1 mg/mL
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Anti-Bone Morphogenetic Protein 4, clone 3H2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | ELISA, IC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Bone Morphogenetic Protein 4, clone 3H2
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for bone morphogenetic protein-4 (BMP-4) by Western blot and ELISA. No cross-reactivity with human BMP-2 or human TGF-β1. The antibody reacts with both human and mouse BMP-4 in both reduced and nonreduced conditions.
Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).
Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).
Molecular Weight:
17 kDa
Immunogen:
Recombinant mouse BMP-4 (Masuhara, 1995).
Clone:
3H2
Isotype:
IgG2b
Background Information:
Bone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
Species Reactivity:
- Human
- Mouse
- Rat
Application Notes:
Immunocytochemistry using osteoinductive cells; i.e., BFP and Saos-2
Western blot using osteoinductive cells; i.e., BFP and Saos-2
Optimal working dilutions must be determined by the end user.
Western blot using osteoinductive cells; i.e., BFP and Saos-2
Optimal working dilutions must be determined by the end user.
Control:
Mouse brain extract
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BMP4
- BMP2B1
- BMP2B
- BMP-4
- DVR4
- BMP-2B
- ZYME
Alternate Names:
BMP-4; DVR-4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunocytochemistry
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
UniProt Summary:
FUNCTION: SwissProt: P12644 # Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair.
SIZE: 408 amino acids; 46555 Da
SUBUNIT: Homodimer; disulfide-linked (By similarity). Interacts with GREM2 (By similarity) and SOSTDC1. Part of a complex consisting of TWSG1 and CHRD (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
SIMILARITY: SwissProt: P12644 ## Belongs to the TGF-beta family.
SIZE: 408 amino acids; 46555 Da
SUBUNIT: Homodimer; disulfide-linked (By similarity). Interacts with GREM2 (By similarity) and SOSTDC1. Part of a complex consisting of TWSG1 and CHRD (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
SIMILARITY: SwissProt: P12644 ## Belongs to the TGF-beta family.
Stem Cell Type:
- Mesenchymal Stem Cells
- Neural Stem Cells
Stem Cell Workflow Stage:
Characterization
Brand Family:
Chemicon
Product Name:
Anti-Bone Morphogenetic Protein 4, clone 3H2
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse