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Ordering Information
- : MAB11015
- : 100 μg
- : 1 mg/mL
Anti-PABPC4, clone 6E1.2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-PABPC4, clone 6E1.2
Clone:
clone 6E1.2
Trade Name:
Upstate (Millipore)
Qty/Pk:
100 μg
Background Information:
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 (Poly(A) binding protein, cytoplasmic 4), also known as IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. PABPC4 may be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may be involved in the regulation of protein translation in platelets and megakaryocytes and may participate in the binding or stabilization of polyadenylates in platelet dense granules.
Key Applications:
Western Blotting
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for
regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.
regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.
Molecular Weight:
~70 kDa
Quality Assurance:
Routinely evaluated by immunoblot.
Immunogen:
Immunogen was a KLH-conjugated, synthetic peptide corresonding to a.a. 416-427 of human PABPC4, between the RRM (RNA recognition motif) 4 and PABC domains. An additional C-terminal cysteine was added for coupling to KLH
Brand Family:
Upstate
Format:
Purified
Presentation:
Immunoaffinity purified rabbit IgG in 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl, 0.05% sodium azide.
UniProt Number:
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Host:
Mouse
Isotype:
IgG1κ
Epitope:
a.a. 416-427
Product Name:
Anti-PABPC4, clone 6E1.2
Entrez Gene Number:
Alternate Names:
- poly(A) binding protein, cytoplasmic 4 (inducible form)
- Inducible poly(A)-binding protein
- Activated-platelet protein 1
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein G purfied
Specificity:
Recognizes human PABPC4