Anti-Collagen Types I, II & III, clone MMCHABC
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, RIA, IH, not WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Collagen Types I, II & III, clone MMCHABC
Trade Name:
Chemicon (Millipore)
Specificity:
Human collagen types I, II and III (both native and denatured). No cross reactivity with human plasma proteins, other collagen types or non-collagenous proteins in ELISA, RIA or immunoblot.
Immunogen:
Human collagen type I
Clone:
MMCHABC
Isotype:
IgM
Species Reactivity:
Human
Application Notes:
ELISA: Apparent binding constant from titration curves is 0.1 nM. Can be used as capture or reporter antibody for determination of collagen I or III in sandwich ELISA, but requires combination with collagen type-specific antibody for other function.
Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.
Not suitable for use on paraffin embedded sections or immunoblots.
Optimal working dilution must be determined by the end user.
Immunohistochemistry: cryostat sections fixed with acetone or ethanol. Staining pattern similar to polyclonal antibody to interstitial collagens. Formaldehyde fixation is not recommended.
Not suitable for use on paraffin embedded sections or immunoblots.
Optimal working dilution must be determined by the end user.
Applications Not Recommended:
Western Blotting
Presentation:
Purified from murine ascites by PEG precipitation and Sepharose™ CL-4B chromatography. Presented as a liquid in 0.1M NaCl, 0.01M Na-phosphate, 0.01M Na-borate, pH7.5 with 0.1% mannitol and 0.1% dextran.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- COL1A1
- OI4
Alternate Names:
Interstitial Collagens
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
Key Applications:
- ELISA
- Radioimmunoassay
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P02461 # Collagen type III occurs in most soft connective tissues along with type I collagen.
SIZE: 1466 amino acids; 138564 Da
SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
DISEASE: SwissProt: P02461 # Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.
SIMILARITY: SwissProt: P02461 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.
SIZE: 1466 amino acids; 138564 Da
SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
DISEASE: SwissProt: P02461 # Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.
SIMILARITY: SwissProt: P02461 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Protein/Isoform Description:
Collagens are proteins in the Extracellular Matrix (ECM), critical in cell adhesion. At least 17 types of Collagen have been identified, with many genes encoding distinct subunits. Collagen type I is found in several tissues including skin, connective tissue, cartilage and bone. Collagen type II is also found in cartilage, as well as in the vitreous humor of the eye. Collagen type III is found in connective tissue, often in association with type I Collagen. These three Collagens, known as fibrillar or interstitial Collagens, are trimers with a triple helical structure; helices assemble into rods and fibrils held together by inter-trimer crosslinks. Disruptions in expression of these Collagens result in several diseases, such as Ehlers-Danlos syndrome (type I and III), osteoporosis (type I), achondrogenesis or chondrodysplasia (type II), osteoarthritis (type II), or aneurysms (type III). Introduction of antibodies against Collagen type II into mice provide a model for rheumatoid arthritis.
Product Name:
Anti-Collagen Types I, II & III, clone MMCHABC
Concentration:
1.7 mg/mL
Antibody Type:
Monoclonal Antibody