Ordering Information
- : MAB13429
- : 100 µg
- : 200 μg/mL
Anti-TIMP-1, clone 102D1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-TIMP-1, clone 102D1
Trade Name:
Chemicon (Millipore)
Specificity:
It recognizes a glycoprotein of 28.5 kDa which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1). TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Cellular Localization:
Cytoplasmic
Cellular Localization:
Cytoplasmic
Immunogen:
Recombinant human TIMP-1.
Clone:
102D1
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Western Blotting: 1-2 μg/mL for 2 hours at room temperature
Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues: 1-2 μg/mL for 30 min. at room temperature.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: Formalin-fixed, paraffin-embedded tissues: 1-2 μg/mL for 30 min. at room temperature.
Optimal working dilutions must be determined by end user.
Control:
POSITIVE CONTROL: Cytotrophoblastic columns, the endothelial and fibroblastic stromal cells of villi, and the large decidualized cells of decidual membrane in placenta1.
Presentation:
Purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TIMP1
- TIMP
- FLJ90373
- CLGI
- EPA
- EPO
- TIMP-1
- HCI
Alternate Names:
Tissue Inhibitor of Metalloproteinase-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
UniProt Summary:
FUNCTION: SwissProt: P01033 # Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
SIZE: 207 amino acids; 23171 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P01033 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
SIZE: 207 amino acids; 23171 Da
SUBCELLULAR LOCATION: Secreted.
PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.
SIMILARITY: SwissProt: P01033 ## Belongs to the protease inhibitor I35 (TIMP) family. & Contains 1 NTR domain.
Brand Family:
Chemicon
Product Name:
Anti-TIMP-1, clone 102D1
Concentration:
200 μg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse