Anti-Actin, Clone C4

P68133

FUNCTION: SwissProt: P68133 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 377 amino acids; 42051 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: SwissProt: P68133 # Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. Nemaline myopathy (NEM) is a form of congenital myopathy characterized by abnormal thread- or rod-like structures in muscle fibers on histologic examination. The clinical phenotype is highly variable, with differing age at onset and severity. & Defects in ACTA1 are a cause of congenital myopathy with excess of thin myofilaments (CM) [MIM:102610]. & Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
SIMILARITY: SwissProt: P68133 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. ... nascondi » visualizza altro »

NM_001100.3

Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

Anti-Actin, near a.a. 50-70, clone C4

Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).

• actin, alpha 1, skeletal muscle
• alpha skeletal muscle actin

C4

MAB1501R is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).

• ELISA
• Immunocytochemistry
• Immunohistochemistry
• Immunohistochemistry (Paraffin)
• Western Blotting

Immunocytochemistry:
10 μg/mL dilution from a previous lot was used (methanol fixed mouse 3T3 cells).

Immunohistochemistry:
10μg/mL dilution from a previous lot was used for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

ELISA:
A previous lot was shown to be strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoidum and Physarum polycephalum.

Western blot:
1-20 µg/ml. On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43 kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha-actin found in skeletal, cardiac, and arterial muscle (Otey, 1987).

Immunohistochemistry:
10µg/mL for paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections {see Luciano, L et al. 2003}.

Optimal working dilutions must be determined by end user.

All

To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.

IgG1κ

Purified chicken gizzard actin (Lessard, 1988).

a.a. 50-70

43 kDa

HeLa whole cell lysate.

Purified mouse monoclonal IgG1k in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.

Stable for 6 months at 2-8°C from date of receipt.

• ACTA1
• MPFD
• ASMA
• NEM1
• CFTDM
• ACTA
• CFTD
• NEM2
• Alpha-actin-1
• CFTD1
• NEM3

Routinely evaluated by Western Blot on HeLa lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected actin on 10 μg of HeLa lysate.

Cell Structure

Cytoskeletal Signaling

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Chemicon (Millipore)

Pur

1 mg/mL

Monoclonal Antibody

Purified

Mouse

Protein A Purified.