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Western Blot Analysis:
Cat. # MAB1503 recognizes Nogo A at 0.1 µg/mL in 20 µg of rat brain Lysate.
Anti-Nogo A, clone 6A5.2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Nogo A, clone 6A5.2
Clone:
6A5.2
Qty/Pk:
100 µg
Background Information:
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Mouse, Human and Rat. Reactivity with other species has not been tested.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq]
UniProt Summary:
FUNCTION: Potent neurite growth inhibitor in vitro and plays a role both in the restriction of axonal regeneration after injury and in structural plasticity in the CNS. May inhibit BACE1 activity and amyloid precursor protein processing (By similarity).
SUBUNIT:Binds to RTN4R. Interacts with Bcl-xl and Bcl-2. Interacts in trans with CNTNAP1 (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains (By similarity).
ALTERNATIVE PRODUCTS: Event=Alternative splicing; Named
isoforms=3; Name=1; IsoId=Q99P72-2; Sequence=Displayed; Name=2; IsoId=Q99P72-3; Sequence=VSP_018089, VSP_018090; Name=3; IsoId=Q99P72-1; Sequence=VSP_018088, VSP_018091.
DOMAIN:Three regions, residues 59-172, 544-725 and the loop 66
amino acids, between the two transmembrane domains, known as
Nogo-66 loop, appear to be responsible for the inhibitory effect on
neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor (By similarity).
SIMILARITY: Contains 1 reticulon domain.
SEQUENCE CAUTION: Sequence=BC056373;Type=Erroneous termination;Positions=721; Note=Translated as Glu.
SUBUNIT:Binds to RTN4R. Interacts with Bcl-xl and Bcl-2. Interacts in trans with CNTNAP1 (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains (By similarity).
ALTERNATIVE PRODUCTS: Event=Alternative splicing; Named
isoforms=3; Name=1; IsoId=Q99P72-2; Sequence=Displayed; Name=2; IsoId=Q99P72-3; Sequence=VSP_018089, VSP_018090; Name=3; IsoId=Q99P72-1; Sequence=VSP_018088, VSP_018091.
DOMAIN:Three regions, residues 59-172, 544-725 and the loop 66
amino acids, between the two transmembrane domains, known as
Nogo-66 loop, appear to be responsible for the inhibitory effect on
neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor (By similarity).
SIMILARITY: Contains 1 reticulon domain.
SEQUENCE CAUTION: Sequence=BC056373;Type=Erroneous termination;Positions=721; Note=Translated as Glu.
Molecular Weight:
130 kDa
Quality Assurance:
Routinely tested on rat brain lysate
Lot Specific Tested Application 1: Western Blot
Lot Specific Tested Application 1: Western Blot
Immunogen:
Recombinant protein
Control:
Rat brain lysate.
Format:
Purified
Presentation:
Purified in PBS with 0.05% NaN3
UniProt Number:
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Host:
Mouse
Isotype:
IgM
Epitope:
Unknown
Gene Symbol:
- ASY
- Foocen
- KIAA0886
- NI220/250
- NOGO
- NOGO-A
- NOGOC
- NSP
- NSP-CL
- Nbla00271
- Nbla10545
- Nogo-B
- Nogo-C
- RTN-X
- RTN-x
- RTN4-A
- RTN4-B1
- RTN4-B2
- RTN4-C
- Reticulon-5
- foocen
Product Name:
Anti-Nogo A, clone 6A5.2
Entrez Gene Number:
Alternate Names:
- Human NogoA
- My043 protein
- Neurite outgrowth inhibitor
- Neuroendocrine-specific protein
- Neuroendocrine-specific protein C homolog
- Nogo protein
- neurite growth inhibitor 220
- reticulon 4
- reticulon 5
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Ammonium sulfate precipitation
Specificity:
Cat. # MAB1503 recognizes Nogo A.