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Ordering Information
- : MAB1510
- : 100 µL
- : Variable
Product Images
Mouse anti-Ubiquitin (Catalog Number MAB1510) staining of dystrophic neurites in temporal cortex of full blown Alzheimer's disease brain.
Mouse anti-Ubiquitin (Catalog Number MAB1510). Alzheimer's disease paired helical filament; gold labeled.
Anti-Ubiquitin, clone Ubi-1 (aka 042691GS)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH(P), IP, WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Ubiquitin, clone Ubi-1 (aka 042691GS)
Trade Name:
Chemicon (Millipore)
Specificity:
Ubiquitin (76 amino acid, 8.5 kDa) both conjugated and unconjugated. Shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson’s disease) and Pick bodies (in Pick’s disease).
Molecular Weight:
8.5 kDa
Immunogen:
Purified ubiquitin (bovine RBC) covalently coupled to KLH using glutaraldehyde.
Clone:
Ubi-1 (aka 042691GS)
Isotype:
IgG1κ
Background Information:
Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Expected to cross- react with Bovine, Chicken, and Drosophila. Reactivity with other species has not been confirmed.
Application Notes:
Immunohistochemistry (frozen or paraffin embedded sections):
1:250-1:1,000 dilution from a previous lot was used.
Electron Microscopy:
1:250-1:1,000 dilution from a previous lot was used.
Immunoprecipitation:
(Liang, 1995; Oberdorf, 1999)
Note: Suggested blocking agent is bovine serum albumin.
Immunoblotting:
1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).
Optimal working dilutions must be determined by end user.
1:250-1:1,000 dilution from a previous lot was used.
Electron Microscopy:
1:250-1:1,000 dilution from a previous lot was used.
Immunoprecipitation:
(Liang, 1995; Oberdorf, 1999)
Note: Suggested blocking agent is bovine serum albumin.
Immunoblotting:
1:250-1:1,000. The sensitivity of the assay can be enhanced by autoclaving the blot (Swerdlow, 1986).
Optimal working dilutions must be determined by end user.
Control:
Alzheimer's diseased brain.
Quality Assurance:
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis:
1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected UBIQUITIN on 10 μg of Jurkat3 lysates.
Purification Method:
Unpurified
Presentation:
Mouse monoclonal IgG1κ in buffer containing containing 10 mM sodium azide.
Storage Conditions:
Stable for 1 year at -20°C in undiluted aliquotes from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RPS27A
- UBA80
- UBC
- CEP80
- Uba80
- UBCEP80
- UBCEP1:UBA52
- UBB
- HUBCEP80
- UBCEP2
Alternate Names:
- Ubiquitin
- ubiquitin-CEP80
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
UniProt Summary:
FUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
SIZE: 76 amino acids; 8565 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIZE: 76 amino acids; 8565 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Brand Family:
Chemicon
Product Name:
Anti-Ubiquitin, clone Ubi-1 (aka 042691GS)
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse