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Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter...
Anti-Myelin Associated Glycoprotein, clone 513
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| B, Ch, H, M, R, Fg | IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: SwissProt: P20916 # Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3- linked sialic acid (By similarity).
SIZE: 626 amino acids; 69069 Da
SUBUNIT: Binds to RTN4R (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P20916 ## Belongs to the immu ... En voir davantage »
SIZE: 626 amino acids; 69069 Da
SUBUNIT: Binds to RTN4R (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P20916 ## Belongs to the immu ... En voir davantage »
Code GenBank:
Entrez Gene Summary
The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
Description:
Anti-Myelin Associated Glycoprotein, clone 513
Background Information:
Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.
Alternate Names:
MAG
Clone:
513
Specificity:
Detects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Application Notes:
Western Blot:
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}
Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.
Optimal working dilutions must be determined by end user.
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}
Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Rat
- Frog
Species Reactivity Note:
Human, rat, mouse, bovine, chicken, frog.
Isotype:
IgG1
Immunogen:
L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.
Molecular Weight:
100 kDa
Control:
Rat hippocampus tissue, rat brain lysate, PC12 cells.
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- MAG
- SIGLEC4A
- SIGLEC-4A
- Siglec-4a
- S-MAG
- GMA
Quality Assurance:
Evaluated by immunohistochemistry on rat hippocampus.
Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Neuronal & Glial Markers
- Neurochemistry & Neurotrophins
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified