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  • : MAB1637
  • : 100 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Product Family Information

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Mouse anti-Beta III Tubulin (Catalog Number MAB1637). Localization of beta III Tubulin (red) and GFAP (green) in rat brain cortical cells thawed and c...

Mouse anti-beta III tubulin (Catalog Number MAB1637). Localization of beta III tubulin in rat brain cortical cells thawed and cultured for 7 days. The...

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Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

Recommended Replacement for: CBL412
Species Reactivity Key Applications Host Format Antibody Type
Av, B, H, M, Mk, Po, R, Sh ELISA, ICC, IHC, IH(P), IP, WB Mouse Ascites Monoclonal Antibody
Description:
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
Replaces:
CBL412
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
Molecular Weight:
55 kDa
Epitope:
C-terminus
Immunogen:
A synthetic peptide corresponding to amino acids 443-450 (ESESQGPK) of human class III beta-tubulin conjugated to KLH.
Clone:
TU-20 (Similar to TUJ1)
Isotype:
Background Information:
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
Species Reactivity:
  • Avian
  • Bovine
  • Human
  • Mouse
  • Monkey
  • Pig
  • Rat
  • Sheep
Species Reactivity Note:
Mouse. Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
Application Notes:
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.

Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

ELISA:
A previous lot of this antibody was used on ELISA.

Optimal working dilution must be determined by the end user.
Control:
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
Purification Method:
Unpurified
Presentation:
Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Storage Conditions:
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • TUBB3
  • TUBB4
  • beta-4
  • MC1R
Alternate Names:
  • Tubulin beta-III
  • tubulin, beta 3
  • tubulin, beta, 4
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.
Brand Family:
Chemicon
Product Name:
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Target / Localization:
All neurons, soma, axons, cytoplasmic, neurites
Format:
Ascites
Host:
Mouse
Applications:
Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB.

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