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Mouse anti-Beta III Tubulin (Catalog Number MAB1637). Localization of beta III Tubulin (red) and GFAP (green) in rat brain cortical cells thawed and c ...
Mouse anti-beta III tubulin (Catalog Number MAB1637). Localization of beta III tubulin in rat brain cortical cells thawed and cultured for 7 days. The ...
Anti-Tubulin, β III isoform, C-terminus, clone TU-20 (Similar to TUJ1)
Recommended Replacement for: CBL412| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, B, H, M, Mk, Po, R, Sh | ELISA, IP, WB, IC, IH, IH(P) | Mouse | Ascites | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.
SIZE: 450 amino acids; 50433 Da
SUBUNIT: Dimer of alpha and beta chains.
DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
SIMILARITY: Belongs to the tubulin family.
Entrez Gene Number:
Description:
Anti-Tubulin, β III isoform, C-terminus, clone TU-20 (Similar to TUJ1)
Replaces:
CBL412
Background Information:
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
Clone:
TU-20 (Similar to TUJ1)
Specificity:
Reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunocytochemistry on fixed cells: 1:100 to 1:500.
Immunohistochemistry on formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoblotting: 1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
Immunoprecipitation
ELISA
Optimal working dilution must be determined by the end user.
Immunohistochemistry on formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoblotting: 1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
Immunoprecipitation
ELISA
Optimal working dilution must be determined by the end user.
Species Reactivity:
- Avian
- Bovine
- Human
- Mouse
- Monkey
- Pig
- Rat
- Sheep
Isotype:
IgG1
Immunogen:
A synthetic peptide corresponding to amino acids 443-450 (ESESQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope:
C-terminus
Molecular Weight:
55 kDa
Control:
Positive Control: Brain tissue or neurons of any type
Negative Control: Non-neural tissue or astrocytes
Negative Control: Non-neural tissue or astrocytes
Presentation:
Liquid in PBS containing 1% BSA and 15 mM sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- TUBB3
- TUBB4
- beta-4
- MC1R
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Neuronal & Glial Markers
- Neurofilament & Neuron Metabolism
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
Variable
Format Code:
Asc
Trade Name:
Chemicon (Millipore)
Purification Method:
UnPurified
Format:
Ascites
Antibody Type:
Monoclonal Antibody
Host:
Mouse