Anti-cAMP-Dependent Protein Kinase II, Regulatory Subunit α/β, clone RS30
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, Po | ELISA, IF, WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P13861 # Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
SIZE: 404 amino acids; 45518 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.
TISSUE SPECIFICITY: Four ty ... see more »
SIZE: 404 amino acids; 45518 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.
TISSUE SPECIFICITY: Four ty ... see more »
Entrez Gene Number:
Entrez Gene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different
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Description:
Anti-cAMP-Dependent Protein Kinase II, Regulatory Subunit α/β, clone RS30
Clone:
RS30
Specificity:
Regulatory subunit of cAMP-dependant protein kinase type II, alpha and beta.
Key Applications:
- ELISA
- Immunofluorescence
- Western Blotting
Application Notes:
Localization of regulatory subunit in normal and tumor cells.
Western blot
Immunofluorescence
ELISA:Bovine1:1,500,000
Porcine1:240,000
Human1:1,000
Optimal working dilutions must be determined by the end user.
Western blot
Immunofluorescence
ELISA:Bovine1:1,500,000
Porcine1:240,000
Human1:1,000
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Bovine
- Human
- Pig
Isotype:
IgG2a
Immunogen:
Purified regulatory subunit of cAMP-dependant protein kinase type II from bovine brain
Epitope:
Regulatory Subunit alpha/beta
Presentation:
Purified immunoglobulin. Liquid in PBS pH 7.4 containing 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain refrigerated at +2-8°C for up to 6 months.
Gene Symbol:
- PRKAR2A
- MGC3606
- PKR2
- PRKAR2
Antibody Category:
Cell Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Concentration:
2.3 mg/ml
Descriptive Text:
Protein kinase A is perhaps the prototype Ser/Thr kinase, which is activated by the second messenger cyclic AMP (cAMP). The PKA holoenzyme consists of two catalytic subunits complexed with two regulatory subunits. In addition, the regulatory subunits bind various AKAP proteins, which localize the holoenzyme to specific locations within the cell. Binding of cAMP to the regulatory subunits results in release of catalytic subunits, which phosphorylate an extremely broad range of substrates, including the transcription factor CREB. PKA activity in a lysate can be neutralized with the pseudosubstrate PKI.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Antibody Type:
Monoclonal Antibody
Host:
Mouse