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Ordering Information
- : MAB1697
- : 100 µg
- : 2.3 mg/ml
Anti-cAMP-Dependent Protein Kinase II, Regulatory Subunit α/β, clone RS30
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, Po | ELISA, IF, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-cAMP-Dependent Protein Kinase II, Regulatory Subunit α/β, clone RS30
Trade Name:
Chemicon (Millipore)
Specificity:
Regulatory subunit of cAMP-dependant protein kinase type II, alpha and beta.
Epitope:
Regulatory Subunit alpha/beta
Immunogen:
Purified regulatory subunit of cAMP-dependant protein kinase type II from bovine brain
Clone:
RS30
Isotype:
IgG2a
Species Reactivity:
- Bovine
- Human
- Pig
Application Notes:
Localization of regulatory subunit in normal and tumor cells.
Western blot
Immunofluorescence
ELISA:Bovine1:1,500,000
Porcine1:240,000
Human1:1,000
Optimal working dilutions must be determined by the end user.
Western blot
Immunofluorescence
ELISA:Bovine1:1,500,000
Porcine1:240,000
Human1:1,000
Optimal working dilutions must be determined by the end user.
Presentation:
Purified immunoglobulin. Liquid in PBS pH 7.4 containing 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain refrigerated at +2-8°C for up to 6 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRKAR2A
- MGC3606
- PKR2
- PRKAR2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER).
Key Applications:
- ELISA
- Immunofluorescence
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P13861 # Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
SIZE: 404 amino acids; 45518 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.
TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
PTM: Phosphorylated by the activated catalytic chain.
SIMILARITY: SwissProt: P13861 ## Belongs to the cAMP-dependent kinase regulatory chain family. & Contains 2 cyclic nucleotide-binding domains.
SIZE: 404 amino acids; 45518 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.
TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
PTM: Phosphorylated by the activated catalytic chain.
SIMILARITY: SwissProt: P13861 ## Belongs to the cAMP-dependent kinase regulatory chain family. & Contains 2 cyclic nucleotide-binding domains.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-cAMP-Dependent Protein Kinase II, Regulatory Subunit α/β, clone RS30
Concentration:
2.3 mg/ml
Antibody Type:
Monoclonal Antibody