Anti-Integrin α4, clone P1H4, azide free
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Pm | ELISA, FC, IC, IH, IP, FUNC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Integrin α4, clone P1H4, azide free
Trade Name:
Chemicon (Millipore)
Specificity:
The involvement of integrins in vascular proliferation, adhesion, and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits Monoclonal antibody MAB16983Z was produced by immunization with human T lymphocytes. The antibody is reactive with integrin alpha-4 from primate and human species.
Immunogen:
Human T lymphocytes
Clone:
P1H4
Isotype:
IgG1
Species Reactivity:
- Human
- Primate
Application Notes:
Immunohistochemistry (preferred fixatives are acetone and alcohols)
Immunocytochemistry
Immunoprecipitation
FACS Analysis
ELISA
Radioimmunoassay
Biological Activity: MAB16983Z inhibits CS-1 and VCAM binding.
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunoprecipitation
FACS Analysis
ELISA
Radioimmunoassay
Biological Activity: MAB16983Z inhibits CS-1 and VCAM binding.
Optimal working dilutions must be determined by end user.
Control:
Widely expressed, Intestinal mucosa
Purification Method:
Protein A purfied
Presentation:
Protein A Purified immunoglobulin in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ITGAM
- MO1A
- CD11b
- CR3A
- MAC1A
- CD11B
- MGC117044
- MAC-1
Alternate Names:
CD49d
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Affects Function
Entrez Gene Summary:
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable
UniProt Summary:
FUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
SIZE: 1152 amino acids; 127179 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
SIZE: 1152 amino acids; 127179 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
Brand Family:
Chemicon
Product Name:
Anti-Integrin α4, clone P1H4, azide free
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse