Anti-Integrin α3, clone P1B5, azide free
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, IH(P), FUNC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Integrin α3, clone P1B5, azide free
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with Human alpha3 integrin.
Clone:
P1B5
Isotype:
IgG1
Background Information:
Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. The alpha-3 subunit (CD49c) is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. The alpha-3 subunit exists in two different splice variants, denoted as "A" and "B". The only difference that results from this differential splicing is a total change in the cytoplasmic domain, while the extracellular domain stays the same. Knock-out mice lacking this subunit show prenatal lethality and abnormalities in the kidneys.
Species Reactivity:
Human
Application Notes:
Suitable for use in attachment inhibition assays using fibroblasts, most epithelial cells, activated lymphocytes on laminin, collagen and fibronectin.
Immunohistochemistry on human tonsil
fresh frozen tissue
paraffin embedded tissue; protease digestion required:
Optimal working dilutions must be determined by end user.
Immunohistochemistry on human tonsil
fresh frozen tissue
paraffin embedded tissue; protease digestion required:
Optimal working dilutions must be determined by end user.
Control:
Human foetal foreskin fibroblasts (HFFF), neuroblastomas
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with no preservatives.
Storage Conditions:
Maintain at 2–8°C in undiluted aliquots for up to 6 months after date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ITGA3
- GAP-B3
- CD49c
- FLJ34704
- MSK18
- VLA3a
- VL3A
- VCA-2
- FLJ34631
- FRP-2
- GAPB3
- CD49C
Alternate Names:
CD49c
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
- Affects Function
Entrez Gene Summary:
ITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular-matrix proteins. The alpha 3 beta 1 integrin is known variously as: very late (activation) antigen 3 ('VLA-3'), very common antigen 2 ('VCA-2'), extracellular matrix receptor 1 ('ECMR1'), and galactoprotein b3 ('GAPB3').
UniProt Summary:
FUNCTION: SwissProt: P26006 # Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Alpha- 3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration.
SIZE: 1066 amino acids; 118698 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
SIZE: 1066 amino acids; 118698 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-3 associates with beta-1. Interacts with HPS5.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform alpha-3A is widely expressed. Isoform alpha-3B is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform alpha-3A is strongly expressed on vascular smooth muscle cells, isoform alpha-3B is detected only on endothelial vein cells.
PTM: Isoform alpha-3A, but not isoform alpha-3B, is phosphorylated on serine residues. Phosphorylation increases after phorbol 12- myristate 13-acetate stimulation.
SIMILARITY: SwissProt: P26006 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Brand Family:
Chemicon
Product Name:
Anti-Integrin α3, clone P1B5, azide free
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse