Ordering Information

Catalogue Number : MAB1956Z
Qty/Pk : 100 μg
Conc. : 1 mg/mL

Anti-Integrin α5, clone P1D6, azide free

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	IH, FUNC	Mouse	Purified	Monoclonal Antibody

Description:

Trade Name:

Chemicon (Millipore)

Specificity:

Reacts with Human alpha 5 integrin. Reactivity with other species is unknown.

Clone:

P1D6

Isotype:

IgG3

Background Information:

Integrin alpha 5 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell surface mediated signalling.

Species Reactivity:

Human

Application Notes:

MAB1956Z is suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T & B lymphocytes on fibronectin.

MAB1956Z is not reactive with the denatured alpha 5 subunit. For immunoblotting, a dot blot is suggested as an alternative to western blotting.

Control:

Widely expressed, ovary

Purification Method:

Protein A purfied

Presentation:

Purified mouse monoclonal in 0.01M Phosphate, 150mM NaCl, pH 7.4, containing no preservatives.

Storage Conditions:

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

UniProt Number:

P08648

Entrez Gene Number:

NM_002205.2

Gene Symbol:

ITGA5
VLA-5
CD49e
VLA5A
FNRA

Alternate Names:

CD49e

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications:

Immunohistochemistry
Affects Function

Entrez Gene Summary:

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

UniProt Summary:

FUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1049 amino acids; 114536 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.

Brand Family:

Chemicon

Product Name:

Anti-Integrin α5, clone P1D6, azide free

Concentration:

1 mg/mL

Antibody Type:

Monoclonal Antibody

Qty/Pk:

100 μg

Format:

Purified