Anti-Integrin αVβ5, clone P1F6, azide free
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H | FC, IH, FUNC, not IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Integrin αVβ5, clone P1F6, azide free
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with the human integrin alphaVbeta5.
Immunogen:
Human UCLA P3 cells (lung carcinoma)
Clone:
P1F6
Isotype:
IgG1
Background Information:
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Species Reactivity:
- Chicken
- Human
Application Notes:
Inhibition of attachment of HT29 cells to vitronectin: >1:1,000
Inhibition of attachment of carcinoma and melanoma cells to vitronectin
Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)
Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)
Immunoprecipitation
FACS
EIA
Optimal working dilutions must be determined by the end user.
Inhibition of attachment of carcinoma and melanoma cells to vitronectin
Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)
Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)
Immunoprecipitation
FACS
EIA
Optimal working dilutions must be determined by the end user.
Applications Not Recommended:
Immunohistochemistry (Paraffin)
Control:
Epithelium, keratinocytes
Purification Method:
Protein A purfied
Presentation:
Purified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
- VNRA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Affects Function
Entrez Gene Summary:
ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
UniProt Summary:
FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Brand Family:
Chemicon
Product Name:
Anti-Integrin αVβ5, clone P1F6, azide free
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse