Anti-Integrin α1, clone FB12
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IC, IH, IP, FUNC, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Integrin α1, clone FB12
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
Molecular Weight:
130 kDa
Clone:
FB12
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation.
Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2μg/mL (Sato et al., 2005).
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2μg/mL (Sato et al., 2005).
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Control:
Microvascular endothelium
Purification Method:
Protein A purfied
Presentation:
Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ITGA1
- CD49a
- VLA1
- VLA-1
Alternate Names:
CD49a
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Affects Function
- Western Blotting
Entrez Gene Summary:
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
UniProt Summary:
FUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen.
SIZE: 1179 amino acids; 130848 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
SIZE: 1179 amino acids; 130848 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
Brand Family:
Chemicon
Product Name:
Anti-Integrin α1, clone FB12
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse