Ordering Information
- : MAB2021Z
- : 500 µL
Anti-Integrin αV, extracellular, clone AV1, azide free
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, WB, FUNC | Mouse | Culture Supernatant | Monoclonal Antibody |
Description:
Anti-Integrin αV, extracellular, clone AV1, azide free
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for human integrin alphaV (other species not tested).
Epitope:
extracellular, azide free
Immunogen:
Human integrin alphaVbeta3 heterodimer isolated from placenta.
Clone:
AV1
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Flow cytometry: 1:5-1:50
Western Blot. Under non-reducing conditions recognizes a band at approximately 165 kDa corresponding to integrin alphaV. Does not recognize the reduced protein.
Function-blocking of integrin alphaV-mediated adhesion to ECM proteins. (1:5-1:20, in HEPES buffer pH 7.4 containing 1mM Mn++ and 1mM Mg++).
Optimal working dilutions and protocols must be determined by end user.
Western Blot. Under non-reducing conditions recognizes a band at approximately 165 kDa corresponding to integrin alphaV. Does not recognize the reduced protein.
Function-blocking of integrin alphaV-mediated adhesion to ECM proteins. (1:5-1:20, in HEPES buffer pH 7.4 containing 1mM Mn++ and 1mM Mg++).
Optimal working dilutions and protocols must be determined by end user.
Presentation:
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ITGAV
- DKFZp686A08142
- MSK8
- CD51
- VNRA
Alternate Names:
CD51; Vitronectin Receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
Key Applications:
- Flow Cytometry
- Western Blotting
- Affects Function
UniProt Summary:
FUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
SIZE: 1048 amino acids; 116038 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Qty/Pk:
500 µL
Brand Family:
Chemicon
Format:
Culture Supernatant
Host:
Mouse
Product Name:
Anti-Integrin αV, extracellular, clone AV1, azide free
Antibody Type:
Monoclonal Antibody