Informazioni per l’ordine
Anti-Disialoganglioside GD3, clone MB3.6
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| H | FC, IF, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Disialoganglioside GD3, clone MB3.6
Clone:
MB3.6
Specificity:
Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunohistochemistry
Application Notes:
Immunohistochemistry (frozen tissue sections)
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Immunofluorescence of cells in culture
Flow cytometry: 1-2 μg per 10E6 cells
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}
Optimal working dilutions must be determined by end user.
Species Reactivity:
Human
Isotype:
IgG3
Immunogen:
FM9 melanoma cells
Presentation:
Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Antibody Category:
Apoptosis & Cancer
Antibody Sub-Category:
Tumor Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Concentration:
1 mg/ml
Antibody Type:
Monoclonal Antibody