Anti-Disialoganglioside GD3, clone MB3.6

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• Data Sheets
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Data Sheets
• Anti-Disialoganglioside GD3, clone MB3.6 - Data Sheet
• FAQs
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FAQs
What is the best primary antibody dilution buffer? » Answer
At Millipore ,we always recommend to dilute our primary antibody solutions in very simple buffers, usually only TBS or PBS without excess blocking proteins, detergents or other compounds, particularly preservatives such as azide (particularly important if using HRP detections systems: NEVER MIX AZIDE and HRP for the best results as azide is a very powerful inhibitor of HRP]. By keeping the primary antibody simple, this increase antibody-antigen interaction and can increase staining, and when sample has been properly blocked and prepared we have seen increase in background, and often see an improvement in the signal to noise ratio with simple, dilute primary antibody dilution buffers.

Does this product react with traditionally fixed, paraffin embedded tissues? » Answer
The product may work with traditional paraffin tissues, but it has not been tested. Please contact our Technical Service Department. For qualifying customers, it may be possible to perform a product a evaluation for paraffin and receive a 50% REBATE if you complete our product evaluation form and agree to share your test results with us (even if the experiment does not work).

• MSDS
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MSDS
• Phosphate Buffered Saline with Sodium Azide and Antibodies
• References
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References
• Biosynthesis and expression of the disialoganglioside GD2, a relevant target antigen on small cell lung carcinoma for monoclonal antibody-mediated cytolysis.
  Cheresh, D A, et al. (1986) Cancer Res., 46: 5112-8 (1986)

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• Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis.
  Cheresh, D.A. et al. (1985) PNAS, USA, 82(15):5155-5159 (1985)

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• Disialogangliosides GD2 and GD3 Are Involved in the Attachment of Human Melanoma and Neuroblastoma Cells to Extracellular Matrix Proteins
  Cheresh, DA et al. (1986) J Cell Biol, 102:688-696 (1986)

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• Analysis of effector cells in human antibody-dependent cellular cytotoxicity with murine monoclonal antibodies.
  Ortaldo, JR et al. (1987) J Immunol., 138(10):3566-3572 (1987)

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• Increased lysis of melanoma by in vivo-elicited human lymphokine-activated killer cells after addition of antiganglioside antibodies in vitro
  Harel, W et al. (1990) Can Res, 50(19):6311-6315 (1990)

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• Expression of De-N-acetyl-gangliosides in Human Melanoma Cells Is Induced by Genistein or Nocodazole
  Sjoberg, ER et al. (1995) J Biol Chem, 270(7):2921-2930 (1995)

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