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Ordering Information

  • : MAB2137
  • : 100 μg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Anti-Homing Receptor Antibody, clone A020

Species Reactivity Key Applications Host Format Antibody Type
B, Ca, H, M, Po, R, Rb ELISA, FC, ICC, IHC, WB Rat Purified Monoclonal Antibody
Description:
Anti-Homing Receptor Antibody, clone A020
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).
Molecular Weight:
90 kDa
Immunogen:
Purified human blood lymphocyte CD44
Clone:
Isotype:
IgG2b
Species Reactivity:
  • Bovine
  • Canine
  • Human
  • Mouse
  • Pig
  • Rat
  • Rabbit
Application Notes:
ELISA 1:2000

Flow Cytometry :1:1000

Immunofluorescence 1:200

Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

Immunoprecipitation 1:200

Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

Optimal working dilutions must be determined by end user.
Control:
Cells of epithelium and highly expressed by carcinomas
Purification Method:
Protein A purfied
Presentation:
Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • CD44
  • LHR
  • HUTCH-I
  • CDW44
  • CDw44
  • ECMR-III
  • Epican
  • MGC10468
  • CD44R
  • PGP-1
  • MDU2
  • CSPG8
  • Pgp1
  • IN
  • MUTCH-I
  • MIC4
  • MDU3
  • MC56
  • HCELL
Alternate Names:
CD44s; HCAM; Pgp-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
UniProt Summary:
FUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
SIZE: 742 amino acids; 81554 Da
SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.
Brand Family:
Chemicon
Product Name:
Anti-Homing Receptor Antibody, clone A020
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Applications:
Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.

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