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Ordering Information
- : MAB302
- : 100 μg
- : 1 mg/mL
Anti-Glutamine Synthetase, clone GS-6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Sh | ELISA, IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Glutamine Synthetase, clone GS-6
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
Molecular Weight:
45 kDa
Immunogen:
Glutamine synthetase purified from sheep brain.
Clone:
GS-6
Isotype:
IgG2a
Background Information:
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
Species Reactivity:
- Human
- Mouse
- Rat
- Sheep
Application Notes:
Western blot: 1:1,000-1:5,000
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Control:
Rat brain tissue, rat brain cytosolic fraction extract
Purification Method:
Protein A purfied
Presentation:
Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.
Storage Conditions:
Maintain antibody at 2–8°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GLUL
- GLNS
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]
Key Applications:
- ELISA
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P15104 # Catalyzes the conversion of ammonia and L-glutamate to L-glutamine.
SIZE: 373 amino acids; 42064 Da
SUBUNIT: Homooctamer.
SUBCELLULAR LOCATION: Cytoplasm.
DISEASE:SwissProt: P15104 # Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
SIMILARITY: SwissProt: P15104 ## Belongs to the glutamine synthetase family.
SIZE: 373 amino acids; 42064 Da
SUBUNIT: Homooctamer.
SUBCELLULAR LOCATION: Cytoplasm.
DISEASE:SwissProt: P15104 # Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
SIMILARITY: SwissProt: P15104 ## Belongs to the glutamine synthetase family.
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Glutamine Synthetase, clone GS-6
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody