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Ordering Information

  • : MAB3068
  • : 100 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

Mouse anti-Connexin 43 (Catalogue No. MAB3068) staining of Mudpuppy retina.

Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CONNEXIN 43 (1:500 d...

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Anti-Connexin 43 Antibody, CT

Species Reactivity Key Applications Host Format Antibody Type
Ca, Ch, H, M, R ELISA, ICC, IF, IHC, IP, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Connexin 43 Antibody, CT
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Connexin-43. Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts. Also effective for detection of Cx43 in human fibroblasts, rat brain, canine ventricular myocytes, and goldfish astrocytes. The molecular weight range (43-37 kDa) detected in Western blot of mouse 3T3 fibroblasts likely represents post-translational modifications of a 43 kDa primary translation product [Mol. Cell Biol. (1990) 10: 1754].
Molecular Weight:
43-47 kDa
Epitope:
C-terminus
Immunogen:
A synthetic peptide corresponding to positions 252-270 of the native sequence from rat cardiac connexin 43 [NH2-GDLSPSKDCGSPK YAYFNGK-COOH]
Clone:
Isotype:
Background Information:
Connexins are highly homologous proteins that assemble as hexamers (connexons) to form gap junctions, small channels which allow for the passage of ions and other small molecules up to 1,200 daltons between neighboring cells. Gap junctions play an important role in a variety of cellular processes including homeostasis, intercellular metabolic cooperation and synchronization, morphogenesis, cell differentiation, and growth control. The most broadly studied connexins are the 26, 32, 43 and 45 kDa isoforms. Connexin 43 is a 382 a.a. protein belonging to the alpha-type (Group II) subfamily of connexin proteins and possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. Connexin 43 has been detected in a variety of tissues including: atrial and ventricular myocytes, granulose cells, astrocytes, fibroblasts, leptomeningeal cells, brain, lung, smooth muscle, uterine muscle, kidney, lens, epithelium, cornea and testis. Connexin 43 also may have a role in communication between migrating cardiac neural crest cells.
Species Reactivity:
  • Canine
  • Chicken
  • Human
  • Mouse
  • Rat
Species Reactivity Note:
Human, rat, canine, mouse, chick.
Application Notes:
Western blot:
1:500 Recognizes proteins of molecular weight 43-47 kDa on Western blots of mouse fibroblasts.

ELISA:
A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

Immunofluorescence:
A previous lot of this antibody was used in Enzyme-linked Immunosorbent Assay.

Immunohistochemistry:
(2% paraformaldehyde fixation) of 12 μm cryosections of canine Purkinje fibers. 1:250-1:1000 dilution of a previous lot was used with incubation for 16 hours at 4ºC prior to fluorescence detection [Circulation Res. (1993) 72: 1124].

Immunocytochemistry (acetone fixation):
1:50-1:300 dilution of a previous lot was used for fluorescence detection (see Mol. Cell Biol (1990) 10: 1754). More sensitive methods of detection will permit increased antibody dilution.

Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.

Optimal working dilutions must be determined by end user.
Control:
Rat brain lysate
Quality Assurance:
Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected CONNEXIN 43 on 10 μg of Mouse brain lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 liquid in buffer containing aqueous buffered solution containing 50% glycerol, BSA and < 0.09% sodium azide
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • GJA1
  • CX43
  • GJAL
  • Cx43
  • ODD
  • DFNB38
  • ODDD
  • ODOD
  • SDTY3
  • Connexin-43
Alternate Names:
  • Gap junction 43 kDa heart protein
  • connexin 43
  • gap junction protein, alpha 1, 43kDa
  • gap junction protein, alpha 1, 43kDa (connexin 43)
  • gap junction protein, alpha-like
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Entrez Gene Summary:
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations.
UniProt Summary:
FUNCTION: SwissProt: P17302 # One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
SIZE: 382 amino acids; 43008 Da
SUBUNIT: A connexon is composed of a hexamer of connexins. Interacts with SGSM3.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
TISSUE SPECIFICITY: Expressed in the heart and fetal cochlea.
DISEASE: SwissProt: P17302 # Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type III and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances. & Defects in GJA1 may be the cause of syndactyly type III (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected. & Defects in GJA1 a the cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
SIMILARITY: SwissProt: P17302 ## Belongs to the connexin family. Alpha-type (group II) subfamily.
Brand Family:
Chemicon
Product Name:
Anti-Connexin 43 Antibody, CT
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
Applications:
Detect Connexin 43 using this Anti-Connexin 43 Antibody, C-terminus validated for use in ELISA, IC, IF, IH, IP & WB.

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