Anti-Phosphotyrosine, clone 3-365-10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, IP, WB, IC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine, clone 3-365-10
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody binds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50 = 10E-6 M, determined by ELISA). The antibody does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulphated [23-25] cholecystokinine) or Na-benzoate (all substances ELISA-tested at a concentration of 1 mM). The antibody is available as an HRP conjugate in catalog number MAB3115.
Immunogen:
Hapten O-phopshotyramine (Steinhiber, 1990)
Modifications:
Phosphorylation
Clone:
3-365-10
Isotype:
IgG1
Species Reactivity:
All
Application Notes:
Western blot: 0.4-1 μg/mL
Immunoprecipitation: 10 μg/100 μL (see attached protocol)
Immunocytochemistry: 5-10 μg/mL.
ELISA (kinase.phophatase assay; see Cleaveland, 1990)
Affinity Chromatography (see Steinhilber, 1990).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 10 μg/100 μL (see attached protocol)
Immunocytochemistry: 5-10 μg/mL.
ELISA (kinase.phophatase assay; see Cleaveland, 1990)
Affinity Chromatography (see Steinhilber, 1990).
Optimal working dilutions must be determined by end user.
Presentation:
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Storage Conditions:
Maintain at 4°C in undiluted aliquots for up to 6 months. Prevent multiple temperature changes; store tightly closed.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Protein/Isoform Description:
Protein Tyrosine phosphorylation was first described in 1979. The development of the Anti-Phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 is the first and best single monoclonal antibody for the detection of tyrosine phosphorylation. It is the most highly cited (75% of related scientific journal articles over the last three years) and validated phosphotyrosine antibody available. It consistently produces high quality, reproducible data, and has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 18 years. In addition to its unparalleled characterization and applicability, 4G10 is well known as one of the most sensitive anti-phosphotyrosines. Now Millipore is excited to announce that we have taken the sensitivity for phosphotyrosine detection to the next level. To improve on something that hundreds have tried and none have succeeded, we pooled 4G10 with the next most highly regarded Anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allows for a greater level of detection on more substrates that even 4G10 alone was capable of.
Product Name:
Anti-Phosphotyrosine, clone 3-365-10
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody