Anti-Phosphotyrosine Antibody, clone 3-365-10
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, IP, WB, ICC | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Phosphotyrosine, clone 3-365-10
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody binds specifically and with a high affinity to proteins with phosphorylated tyrosine residues. Binding can be blocked by phosphotyrosine and phenyl phosphate (IC50 = 10E-6 M, determined by ELISA). The antibody does not crossreact with tyrosine, phosphoserine, phosphothreonine, sulphotyrosine (sulphated [23-25] cholecystokinine) or Na-benzoate (all substances ELISA-tested at a concentration of 1 mM). The antibody is available as an HRP conjugate in catalog number MAB3115.
Immunogen:
Hapten O-phopshotyramine (Steinhiber, 1990)
Modifications:
Phosphorylation
Clone:
3-365-10
Isotype:
IgG1
Species Reactivity:
All
Application Notes:
Western blot: 0.4-1 μg/mL
Immunoprecipitation: 10 μg/100 μL (see attached protocol)
Immunocytochemistry: 5-10 μg/mL.
ELISA (kinase.phophatase assay; see Cleaveland, 1990)
Affinity Chromatography (see Steinhilber, 1990).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 10 μg/100 μL (see attached protocol)
Immunocytochemistry: 5-10 μg/mL.
ELISA (kinase.phophatase assay; see Cleaveland, 1990)
Affinity Chromatography (see Steinhilber, 1990).
Optimal working dilutions must be determined by end user.
Presentation:
Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Storage Conditions:
Maintain at 4°C in undiluted aliquots for up to 6 months. Prevent multiple temperature changes; store tightly closed.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
Brand Family:
Chemicon
Product Name:
Anti-Phosphotyrosine, clone 3-365-10
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse
Applications:
This Phosphotyrosine antibody is validated for use in ELISA,IP,WB and ICC for the detection of the Phosphotyrosine protein.