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Anti-A2B5, clone A2B5-105
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ma | FC, IC, IF, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-A2B5, clone A2B5-105
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979).
Immunogen:
Embryonic chicken retinal cells
Clone:
A2B5-105
Isotype:
IgM
Species Reactivity:
Mammals
Application Notes:
Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.
Control:
Type II astrocytes, human neural progenitors
Purification Method:
Ammonium sulfate precipitation
Presentation:
Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
A2B5
Alternate Names:
Neuron Cell Surface Antigen
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunofluorescence
- Immunohistochemistry
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-A2B5, clone A2B5-105
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody