Ordering Information
- : MAB3132
- : 200 µg
Anti-Surfactant Protein D, lectin domain, clone IVF11
Recommended Replacement for: MAB3130| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ELISA, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Surfactant Protein D, lectin domain, clone IVF11
Replaces:
MAB3130
Trade Name:
Chemicon (Millipore)
Specificity:
MAB3132 recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells (in which SpD is highly concentrated), SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells are negative. No reaction on rat skin, colon, kidney or liver. SP-A cross reacitivity is typically evident at very low dilutions (1 to less than a few hundred) by ELISA.
Epitope:
lectin domain
Immunogen:
Purified rat surfactant protein D from bronchoalveolar lavage.
Clone:
IVF11
Isotype:
IgG1κ
Background Information:
Surfactant protein D is a Ca2+ dependent carbohydrate binding protein with a structure similar to other C-type mammalian lectins. Its molecular weight is 620 kDa under non-reducing conditions and 43 kDa under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A.
Species Reactivity:
- Human
- Rat
Application Notes:
Immunohistochemistry on snap frozen or paraffin embedded tissue.
Suggested working dilution for fresh rat tissue is 1:400 and for fresh human tissue is 1:20. Fixation: acetone 5 min at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W for paraffin tissues.
Western blot
ELISA: as capture antibody
Optimal working dilutions must be determined by the end user.
Suggested working dilution for fresh rat tissue is 1:400 and for fresh human tissue is 1:20. Fixation: acetone 5 min at 4°C, paraformaldehyde, paraffin. Tissue pretreatment: microwave treatment 2 x 5 min at 750 W for paraffin tissues.
Western blot
ELISA: as capture antibody
Optimal working dilutions must be determined by the end user.
Presentation:
Immunoglobulin purified from tissue culture supernatant. Lyophilized from buffer containing 5 mg/mL BSA and 0.09% sodium azide.
Storage Conditions:
Maintain lyophilized material at 2-8°C for up to 12 months from date of receipt. Reconstitute lyophilized material with 500 μL of sterile distilled water. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SFTPD
- SP-D
- SFTP4
- PSPD
- PSP-D
- COLEC7
Alternate Names:
SP-D
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35247 # Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
SIZE: 375 amino acids; 37698 Da
SUBUNIT: Oligomeric complex of 4 set of homotrimers.
SUBCELLULAR LOCATION: Secreted, extracellular space.
PTM: The N-terminus is blocked. & Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates (By similarity).
SIMILARITY: SwissProt: P35247 ## Contains 1 C-type lectin domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C)."
SIZE: 375 amino acids; 37698 Da
SUBUNIT: Oligomeric complex of 4 set of homotrimers.
SUBCELLULAR LOCATION: Secreted, extracellular space.
PTM: The N-terminus is blocked. & Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates (By similarity).
SIMILARITY: SwissProt: P35247 ## Contains 1 C-type lectin domain. & Contains 1 collagen-like domain.
MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C)."
Qty/Pk:
200 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Surfactant Protein D, lectin domain, clone IVF11
Antibody Type:
Monoclonal Antibody