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Mouse anti-CNPase (Catalog Number MAB326) localization of CNPase in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Anti-CNPase, clone 11-5B
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| B, Ca, H, M, Po, R, Rb, Sh, not Ch, not Gp | IC, IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
UniProt序列号:
UniProt Summary
SIZE: 421 amino acids; 47579 Da
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
GenBank序列号:
Description:
Anti-CNPase, clone 11-5B
Background Information:
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Alternate Names:
2'3'-Cyclic Nucleotide 3'-Phosphohydrolase
Clone:
11-5B
Specificity:
CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Bovine
- Canine
- Human
- Mouse
- Pig
- Rat
- Rabbit
- Sheep
Species Reactivity Note:
Bovine, canine, human, mouse, porcine, rat, rabbit and sheep.
Non-Reactive Species:
- Chicken
- Guinea Pig
Isotype:
IgG1
Immunogen:
Purified human brain CNPase
Molecular Weight:
48 & 46 kDa
Control:
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
Gene Symbol:
- CNP
- CNPase
- CNP1
- EC 3.1.4.37
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1.0 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified