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Mouse anti-CNPase (Catalog Number MAB326) localization of CNPase in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Anti-CNPase, clone 11-5B
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, M, Po, R, Rb, Sh, not Ch, not Gp | IC, IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CNPase, clone 11-5B
Trade Name:
Chemicon (Millipore)
Specificity:
CNPase, 48 and 46 kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc.
Molecular Weight:
48 & 46 kDa
Immunogen:
Purified human brain CNPase
Clone:
11-5B
Isotype:
IgG1
Background Information:
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
Species Reactivity:
- Bovine
- Canine
- Human
- Mouse
- Pig
- Rat
- Rabbit
- Sheep
Species Reactivity Note:
Bovine, canine, human, mouse, porcine, rat, rabbit and sheep.
Non-Reactive Species:
- Chicken
- Guinea Pig
Application Notes:
Immunocytochemistry:
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
A previous lot was used on primary oligodendrocyte cultures.
Immunohistochemistry:
A previous lot was used on rat hippocampus tissue and rat spinal cord.
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections and mixed glial tumors (oligodendrogliomas, etc.) CNPase I (46 kDa) and CNPase II (48 kDa), which are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Immunohistochemistry on both fresh frozen and paraffin embedded tissue (microwave pretreatment, ctirate pH 6.0).
Immunoblot:
Immunoblotting of myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Optimal working dilutions must be determined by end user.
Control:
Western Blot: Fresh bovine whole brain extract, mouse brain lysate.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Immunohistochemistry: Rat hippocampus tissue, rat spinal cord tissue.
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CNPASE on 10 μg of Mouse Brain lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CNP
- CNPase
- CNP1
- EC 3.1.4.37
Alternate Names:
2'3'-Cyclic Nucleotide 3'-Phosphohydrolase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
SIZE: 421 amino acids; 47579 Da
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
Brand Family:
Chemicon
Product Name:
Anti-CNPase, clone 11-5B
Concentration:
1.0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse