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Mouse anti-Oligodendrocytes (Catalog Number MAB328). Staining of Oligodendrocytes from neonatal rat.
Anti-Oligodendrocytes, clone CE-1
| Reactividad de especies | Aplicaciones claves | Huésped | Formato | Tipo de anticuerpo |
|---|---|---|---|---|
| Ch, H, M, Mk, R, Fe | IP, IC, IH, IH(P), no WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Oligodendrocytes, clone CE-1
Alternate Names:
MOSP
Clone:
CE-1
Specificity:
In neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994).
Key Applications:
- Immunoprecipitation
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Applications Not Recommended:
Western Blotting
Application Notes:
Immunocytochemistry
Immunohistochemistry at 1:1,000-1:5000
Not suggested for use in Western blot.
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Immunohistochemistry at 1:1,000-1:5000
Not suggested for use in Western blot.
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Species Reactivity:
- Chicken
- Human
- Mouse
- Monkey
- Rat
- Feline
Isotype:
IgM
Immunogen:
Rat glial membranes and whole brain white matter.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Ascites
Host:
Mouse
Antibody Type:
Monoclonal Antibody