注文に関する情報
画像情報
Mouse anti-GFAP (Catalogue No. MAB3402) localization of GFAP in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Glial Fibrillary Acid...
)
)
)
Anti-Glial Fibrillary Acidic Protein, clone GA5
右記製品の推奨できる代替品: CBL411| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, R, M, Po, Ch, B, Rb | IC, IH, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... もっと見る »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... もっと見る »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein, clone GA5
Replaces:
CBL411
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Alternate Names:
GFAP
Clone:
GA5
Specificity:
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Immunocytochemistry:
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Rat
- Mouse
- Pig
- Chicken
- Bovine
- Rabbit
Species Reactivity Note:
Human, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
Isotype:
IgG1
Immunogen:
Purified glial filament (Debus, E., 1983).
Molecular Weight:
50 kDa
Control:
Mouse brain tissue, Astrocyte culture.
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Storage Conditions:
Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.
DO NOT FREEZE.
Gene Symbol:
- GFAP
- FLJ45472
Quality Assurance:
Routinely evaluated by Western Blot on Mouse brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1.0 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified