Ordering Information
- : MAB3402
- : 40 µg
- : 1.0 mg/mL
Product Images
Mouse anti-GFAP (Catalogue No. MAB3402) localization of GFAP in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Glial Fibrillary Aci...
 localization of GFAP in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.'))
. <br />Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.<br /><br />Arrow indicates protein Glial Fibrillary Acidic Protein (~51 kDa).'))
 and Mouse brain membrane lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Glial Fibrillary Acidic Protein (1:1000 dilution of a previous lot). <br />Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.<br /><br />Arrow indicates protein Glial Fibrillary Acidic Protein (~51 kDa).'))
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB3402
Recommended Replacement for: CBL411| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Po, Ch, B, Rb | ICC, IHC, IH(P), WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB3402
Replaces:
CBL411
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
Molecular Weight:
50 kDa
Immunogen:
Purified glial filament (Debus, E., 1983).
Clone:
GA5
Isotype:
IgG1
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Species Reactivity:
- Human
- Rat
- Mouse
- Pig
- Chicken
- Bovine
- Rabbit
Species Reactivity Note:
Human, mouse, and rat. Expected to cross-react with porcine, chicken, bovine, and rabbit.
Application Notes:
Immunocytochemistry:
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
Control:
Mouse brain tissue, Astrocyte culture.
Quality Assurance:
Routinely evaluated by Western Blot on Mouse brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Storage Conditions:
Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.
DO NOT FREEZE.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GFAP
- FLJ45472
Alternate Names:
GFAP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Entrez Gene Summary:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
UniProt Summary:
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
Brand Family:
Chemicon
Product Name:
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 | MAB3402
Concentration:
1.0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
40 µg
Format:
Purified
Host:
Mouse