Ordering Information

Catalogue Number : MAB341
Qty/Pk : 100 μg
Conc. : 1.0 mg/mL

Anti-GABA A Receptor β 2,3 Chain, extracellular domain of the β2 & β3 subunits of the GABA A receptor, clone BD17

Recommended Replacement for: AB5952

Species Reactivity	Key Applications	Host	Format	Antibody Type
B, H, M, R, Mk, Fe	IH, IP, WB	Mouse	Purified	Monoclonal Antibody

Description:

Replaces:

AB5952

Trade Name:

Chemicon (Millipore)

Specificity:

Reacts with the β-chain of the GABAA receptor.

Molecular Weight:

55 kDa

Epitope:

Extracellular domain of the beta2 & beta3 subunits of the GABAA receptor

Immunogen:

Purified GABA/benzodiazepine receptor from bovine cortex

Clone:

BD17

Isotype:

IgG1

Background Information:

Gamma-aminobutyric acid type A (GABAA) receptors are members of the neurotransmitter ligand-gated ion channels that mediate neuronal inhibition on binding GABA. The effects of GABA on GABAA receptors are modulated by a range of therapeutically important drugs, including barbiturates, anaesthetics and benzodiazepines.

Species Reactivity:

Bovine
Human
Mouse
Rat
Monkey
Feline

Species Reactivity Note:

Expected to react with human and rat based on sequence homology.

Application Notes:

Immunohistochemistry:
A 10-20 μg/mL concetration of a previous lot was used in IH.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
5-20 µg/mL. Recognizes a 55 kDa band corresponding to the beta chain of the GABAA receptor.

Optimal working dilutions must be determined by end user.

Control:

HepG2 cell lysate

Quality Assurance:

Evaluated by Western Blot on mouse brain membrane lysates.

Western Blot:
1:500 dilution of this antibody detected GABA A Receptor β 2,3 Chain on 10 μg of mouse brain membrane lysates.

Purification Method:

Protein A purfied

Presentation:

Purified mouse monoclonal IgG1 liquid in buffer containing 10 mM potassium phosphate buffer pH 7.4 with 150 mM NaCl

Storage Conditions:

Stable for 1 year at -20ºC from date of receipt.

UniProt Number:

P47870

Entrez Gene Number:

NM_000813.2

Gene Symbol:

GABRB2
GABA
MGC119388
MGC119389
MGC119386

Alternate Names:

GABA(A) receptor subunit beta-2
gamma-aminobutyric acid (GABA) A receptor, beta 2
gamma-aminobutyric acid A receptor, beta 2

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications:

Immunohistochemistry
Immunoprecipitation
Western Blotting

Entrez Gene Summary:

The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 2 subunit. It is mapped to chromosome 5q34 in a cluster comprised of genes encoding alpha 1 and gamma 2 subunits of the GABA A receptor. Alternative splicing of this gene generates 2 transcript variants, differing by a 114 bp insertion.

UniProt Summary:

FUNCTION: SwissProt: P47870 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
SIZE: 474 amino acids; 54606 Da
SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P47870 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family

Brand Family:

Chemicon

Product Name:

Anti-GABA A Receptor β 2,3 Chain, extracellular domain of the β2 & β3 subunits of the GABA A receptor, clone BD17

Concentration:

1.0 mg/mL

Antibody Type:

Monoclonal Antibody

Qty/Pk:

100 μg

Format:

Purified