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Mouse anti-MAP2 (Catalogue No. MAB3418). Localization of MAP2 (red) and GFAP (green) in rat Cortical cells thawed and cultured for 7 days. The blue st...
Mouse anti-MAP2 (Cat. No. MAB3418-50UG). Localization of MAP2 (red) in primary rat hippocampus-derived embryonic neurons that have been thawed and cu...
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Anti-MAP2, clone AP20
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R, Xn, Qu | IH, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-MAP2, clone AP20
Trade Name:
Chemicon (Millipore)
Specificity:
MAB3418 binds specifically to MAP-2.
Molecular Weight:
230 kDa
Immunogen:
Bovine brain microtubule protein.
Clone:
AP20
Isotype:
IgG
Background Information:
MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Rat
- Xenopus
- Quail
Species Reactivity Note:
Rat and mouse. Expected to cross-react with human, cow, chicken, quail, and Xenopus.
Application Notes:
Western Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.
Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).
Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working concentrations must be determined by the end user.
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.
Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).
Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.
Optimal working concentrations must be determined by the end user.
Control:
Brain, Neuronal culture, Human glioblastoma T98G cells.
Quality Assurance:
Routinely evaluated by Western Blot on Rat Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MAP2
- MAP2B
- MAP-2
- MAP2C
- DKFZp686I2148
- MAP2A
Alternate Names:
- Microtubule Associated Protein
- MAP2A and 2B
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
UniProt Summary:
FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Brand Family:
Chemicon
Product Name:
Anti-MAP2, clone AP20
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 µg
Format:
Purified
Host:
Mouse
Replaced By:
MAB378