Información pedidos
Anti-Galactocerebroside, clone mGalC
| Reactividad de especies | Aplicaciones claves | Huésped | Formato | Tipo de anticuerpo |
|---|---|---|---|---|
| Ch, B, H, M, R, Rb | ELISA, IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
Número UniProt:
UniProt Summary
FUNCTION: SwissProt: P54803 # Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
SIZE: 669 amino acids; 75147 Da
SUBCELLULAR LOCATION: Lysosome.
TISSUE SPECIFICITY: Highest level of activity in testes ... ver más »
SIZE: 669 amino acids; 75147 Da
SUBCELLULAR LOCATION: Lysosome.
TISSUE SPECIFICITY: Highest level of activity in testes ... ver más »
Número GenBank:
Entrez Gene Summary
This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Description:
Anti-Galactocerebroside, clone mGalC
Background Information:
Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.
Alternate Names:
- GalC
- Galactoceramide
- Galactosylceramidase
- galactocerebrosidase
- GALCERase
Clone:
mGalC
Specificity:
Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.
Key Applications:
- ELISA
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Application Notes:
Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Chicken
- Bovine
- Human
- Mouse
- Rat
- Rabbit
Isotype:
IgG3
Immunogen:
Synaptic plasma membranes from bovine hippocampus
Molecular Weight:
~75 kDa
Control:
Neonatal cortex
Presentation:
Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- GALC
- EC 3.2.1.46
Quality Assurance:
Evaluated by Western Blot on PC12 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Galactocerebroside on 10 µg of PC12 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Galactocerebroside on 10 µg of PC12 lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Format:
Purified
Host:
Mouse
Purification Method:
Protein A Purified