Content Loading...
Content Loading...
Anti-Galactocerebroside, clone mGalC
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, B, H, M, R, Rb | ELISA, IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Galactocerebroside, clone mGalC
Trade Name:
Chemicon (Millipore)
Specificity:
Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.
Molecular Weight:
~75 kDa
Immunogen:
Synaptic plasma membranes from bovine hippocampus
Clone:
mGalC
Isotype:
IgG3
Background Information:
Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.
Species Reactivity:
- Chicken
- Bovine
- Human
- Mouse
- Rat
- Rabbit
Application Notes:
Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
Control:
Neonatal cortex
Quality Assurance:
Evaluated by Western Blot on PC12 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Galactocerebroside on 10 µg of PC12 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Galactocerebroside on 10 µg of PC12 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GALC
- EC 3.2.1.46
Alternate Names:
- GalC
- Galactoceramide
- Galactosylceramidase
- galactocerebrosidase
- GALCERase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
UniProt Summary:
FUNCTION: SwissProt: P54803 # Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
SIZE: 669 amino acids; 75147 Da
SUBCELLULAR LOCATION: Lysosome.
TISSUE SPECIFICITY: Highest level of activity in testes compared to brain, kidney, placenta and liver. Can also be found in urine.
DISEASE: SwissProt: P54803 # Defects in GALC are the cause of globoid cell leukodystrophy (GLD) [MIM:245200]; also known as Krabbe disease. This autosomal recessive disorder results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin. Clinically, the most frequent form is the infantile form. Most patients (90%) present before six months of age with irritability, spasticity, arrest of motor and mental development, and bouts of temperature elevation without infection. This is followed by myoclonic jerks of arms and legs, oposthotonus, hypertonic fits, and mental regression, which progresses to a severe decerebrate condition with no voluntary movements and death from respiratory infections or cerebral hyperpyrexia before 2 years of age. However, a significant number of cases with later onset, presenting with unexplained blindness, weakness and/or progressive motor, and sensory neuropathy that can progress to severe mental incapacity and death, have been identified.
SIMILARITY: SwissProt: P54803 ## Belongs to the glycosyl hydrolase 59 family.
SIZE: 669 amino acids; 75147 Da
SUBCELLULAR LOCATION: Lysosome.
TISSUE SPECIFICITY: Highest level of activity in testes compared to brain, kidney, placenta and liver. Can also be found in urine.
DISEASE: SwissProt: P54803 # Defects in GALC are the cause of globoid cell leukodystrophy (GLD) [MIM:245200]; also known as Krabbe disease. This autosomal recessive disorder results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin. Clinically, the most frequent form is the infantile form. Most patients (90%) present before six months of age with irritability, spasticity, arrest of motor and mental development, and bouts of temperature elevation without infection. This is followed by myoclonic jerks of arms and legs, oposthotonus, hypertonic fits, and mental regression, which progresses to a severe decerebrate condition with no voluntary movements and death from respiratory infections or cerebral hyperpyrexia before 2 years of age. However, a significant number of cases with later onset, presenting with unexplained blindness, weakness and/or progressive motor, and sensory neuropathy that can progress to severe mental incapacity and death, have been identified.
SIMILARITY: SwissProt: P54803 ## Belongs to the glycosyl hydrolase 59 family.
Brand Family:
Chemicon
Product Name:
Anti-Galactocerebroside, clone mGalC
Concentration:
1 .0 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 μg
Format:
Purified
Host:
Mouse