Product Images
Fluorescence intensity histogram plots of SILENUS® FITC conjugated anti-HbF using SILENUS® Foetal Control Kit. The vertical axes have been scaled so ...
Anti-HbF, clone WBAC HbF1, FITC conjugated
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | Mouse | FITC | Monoclonal Antibody |
Description:
Anti-HbF, clone WBAC HbF1, FITC conjugated
Trade Name:
Chemicon (Millipore)
Product Overview:
PRINCIPLE:
This test is confined to use on maternal blood samples from women while pregnant or following delivery where fetal hemorrhage is suspected. FMH during pregnancy may be caused by threatened miscarriage, chorionic villus sampling, placenta abruption, abdominal trauma or trauma induced by external cephalic version. FMH also may occur as a result of ectopic pregnancy, spontaneous abortion or termination of pregnancy. The test should be performed on maternal blood after any such event1. In addition, the magnitude of FMH should be estimated following delivery of Rh D positive infants1. The test should be carried out soon after the precipitating event or delivery in order to permit the timely administration of the appropriate amount of Rh D immunoglobulin to prevent alloimmunisation occurring in the mother.
INTRODUCTION:
Currently, there is a world shortage of Rh D immunoglobulin for immunoprophylaxis. Administration of the appropriate dose is encouraged to ensure adequate protection against alloimmunisation whilst conserving a valuable resource. To determine the appropriate dose, it is necessary to quantitate the magnitude of a suspected FMH and administer Rh D immunoglobulin according to the extent of the fetal bleed. The required dose is estimated on the basis that 20 μg of Rh D immunoglobulin will suppress immunization by 1 mL D-positive red cells (2 mL Fetal blood). Pack sizes for Rh D immunoglobulin vary. In Australia, 125 μg is considered to be sufficient to suppress immunization by up to 12 mL of Fetal blood1. Assuming a maternal blood volume of 5000ml, a hemorrhage of this magnitude would be detectable in the maternal circulation as approximately 0.25% of the red cell population or 1/400 cells. The capacity of the flow cytometer to analyse a large number of cells in a short period of time enables accurate quantitation of FMH at clinically relevant levels. Anti HbF is designed for use with a flow cytometer. The test is simple to perform, rapid, reliable and objective.
This test is confined to use on maternal blood samples from women while pregnant or following delivery where fetal hemorrhage is suspected. FMH during pregnancy may be caused by threatened miscarriage, chorionic villus sampling, placenta abruption, abdominal trauma or trauma induced by external cephalic version. FMH also may occur as a result of ectopic pregnancy, spontaneous abortion or termination of pregnancy. The test should be performed on maternal blood after any such event1. In addition, the magnitude of FMH should be estimated following delivery of Rh D positive infants1. The test should be carried out soon after the precipitating event or delivery in order to permit the timely administration of the appropriate amount of Rh D immunoglobulin to prevent alloimmunisation occurring in the mother.
INTRODUCTION:
Currently, there is a world shortage of Rh D immunoglobulin for immunoprophylaxis. Administration of the appropriate dose is encouraged to ensure adequate protection against alloimmunisation whilst conserving a valuable resource. To determine the appropriate dose, it is necessary to quantitate the magnitude of a suspected FMH and administer Rh D immunoglobulin according to the extent of the fetal bleed. The required dose is estimated on the basis that 20 μg of Rh D immunoglobulin will suppress immunization by 1 mL D-positive red cells (2 mL Fetal blood). Pack sizes for Rh D immunoglobulin vary. In Australia, 125 μg is considered to be sufficient to suppress immunization by up to 12 mL of Fetal blood1. Assuming a maternal blood volume of 5000ml, a hemorrhage of this magnitude would be detectable in the maternal circulation as approximately 0.25% of the red cell population or 1/400 cells. The capacity of the flow cytometer to analyse a large number of cells in a short period of time enables accurate quantitation of FMH at clinically relevant levels. Anti HbF is designed for use with a flow cytometer. The test is simple to perform, rapid, reliable and objective.
Specificity:
This antibody recognizes Fetal Hemaglobin (HbF) found on human red blood cells (RBC).
Clone:
WBAC HbF1
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
This reagent is designed for the detection and quantitation of HbF positive RBC arising in the circulation of mothers as a consequence of fetomaternal hemorrhage (FMH). The procedure requires prefixation and permeabilisation of cells followed by a single staining step and analysis using a flow cytometer.
Presentation:
SILENUS®FITC conjugated anti-HbF is supplied in phosphate buffered saline, pH 7.2, containing 0.2% BSA and 0.1% Sodium Azide.
MATERIALS REQUIRED BUT NOT PROVIDED
Blood collection tubes containing EDTA as anti-coagulant.
Test tubes compatible with the sampling system of the flow cytometer to be used.
Micropipettors.
Vortex mixer.
Benchtop centrifuge suitable for sedimentation of RBC.
Wash buffer, Prefixation, Permeabilisatiion and Fixing solutions (see Reagent Preparation).
Flow cytometer equipped with a laser capable of exciting FITC (488nm wavelength) and a filter configuration suitable for detection of the emitted signals.
MATERIALS REQUIRED BUT NOT PROVIDED
Blood collection tubes containing EDTA as anti-coagulant.
Test tubes compatible with the sampling system of the flow cytometer to be used.
Micropipettors.
Vortex mixer.
Benchtop centrifuge suitable for sedimentation of RBC.
Wash buffer, Prefixation, Permeabilisatiion and Fixing solutions (see Reagent Preparation).
Flow cytometer equipped with a laser capable of exciting FITC (488nm wavelength) and a filter configuration suitable for detection of the emitted signals.
Storage Conditions:
Store at 2-8°C in undiluted aliquots for up to 12 months. DO NOT FREEZE Protect from light.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Flow Cytometry
Qty/Pk:
100 assays
Brand Family:
Chemicon
Format:
FITC
Host:
Mouse
Product Name:
Anti-HbF, clone WBAC HbF1, FITC conjugated
Antibody Type:
Monoclonal Antibody