Ordering Information
- : MAB347
- : 50 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-GAP-43/B-50 (1:1000 di...
Western Blot Analysis:
Representative image from a previous lot.
Mouse brain (Lane1), rat brain (Lane2) and mouse brain membrane lysate (Lan...
Anti-Growth Associated Protein 43, clone 9-1E12
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Fe, Ht | IH, IP, WB | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Growth Associated Protein 43, clone 9-1E12
Trade Name:
Chemicon (Millipore)
Specificity:
GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.
Molecular Weight:
43-48 kDa
Immunogen:
GAP-43 purified from rat brain
Clone:
9-1E12
Isotype:
IgG1
Background Information:
GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
Species Reactivity:
- Human
- Rat
- Feline
- Hamster
Species Reactivity Note:
Rat, feline and hamster.
Expected to react with human based on sequence homology.
Expected to react with human based on sequence homology.
Application Notes:
Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.
Control:
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
Quality Assurance:
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
Maintain at 2-8°C in undiluted for up to 6 months after date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GAP43
- PP46
- B-50
- GAP-43/B50
Alternate Names:
- Neuromodulin
- B-50 Protein
- Growth Associated Protein 43
- neuromodulin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system.
UniProt Summary:
FUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.
Brand Family:
Chemicon
Product Name:
Anti-Growth Associated Protein 43, clone 9-1E12
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
50 µg
Format:
Purified
Host:
Mouse