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Ordering Information
- : MAB3472
- : 100 µL
Anti-Hypoxia Inducible Factor 2 α, clone 190b
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IC, IH(P) | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Hypoxia Inducible Factor 2 α, clone 190b
Trade Name:
Chemicon (Millipore)
Specificity:
Hypoxia inducible factor-2 alpha (HIF-2 alpha).
Immunogen:
Recombinant human HIF-2 alpha.
Clone:
190b
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Western blot on whole cell extracts. Suggested blocking solution is PBS containing 5% non-fat dried milk and 0.1% Tween 20.
Immunohistochemistry on paraffin embedded tissue sections. Recommended antigen retrieval: pressure cooking in 50 mmol/L Tris and 0.2 mmol/L EDTA buffer, pH 9.0 for 180 seconds or incubating at 60°C for 16 hours overnight.
Immunocytochemistry on transfected COS-1 cells (snap frozen in liquid nitrogen or fixed overnight in 10% neutral buffered formalin and paraffin embedded).
ELISA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on paraffin embedded tissue sections. Recommended antigen retrieval: pressure cooking in 50 mmol/L Tris and 0.2 mmol/L EDTA buffer, pH 9.0 for 180 seconds or incubating at 60°C for 16 hours overnight.
Immunocytochemistry on transfected COS-1 cells (snap frozen in liquid nitrogen or fixed overnight in 10% neutral buffered formalin and paraffin embedded).
ELISA
Optimal working dilutions must be determined by the end user.
Presentation:
Ascites fluid. Liquid. Contains no preservative.
Storage Conditions:
Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- EPAS1
- EPAS-1
- HIF2A
- HLF
- MOP2
- PASD2
Alternate Names:
HIF-2 alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: Q99814 # Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
SIZE: 870 amino acids; 96459 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP (By similarity).
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
PTM: In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). & In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation (By similarity). & Phosphorylated on multiple sites in the CTAD (By similarity).
SIMILARITY: SwissProt: Q99814 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.
SIZE: 870 amino acids; 96459 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT. Interacts with CREBBP (By similarity).
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
PTM: In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization (By similarity). & In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation (By similarity). & Phosphorylated on multiple sites in the CTAD (By similarity).
SIMILARITY: SwissProt: Q99814 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Ascites
Host:
Mouse
Product Name:
Anti-Hypoxia Inducible Factor 2 α, clone 190b
Antibody Type:
Monoclonal Antibody