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Ordering Information
- : MAB3520
- : 100 µg
- : 1 mg/mL
Product Images
Mouse anti-Ubiquitin Activating Enzyme E1 (Catalog Number MAB3520) Western blot analysis of bacterially expressed human E1. The lower band is an E1 de...
Anti-Ubiquitin Activating Enzyme E1, clone 2G2.3
Recommended Replacement for: 05-624| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Ubiquitin Activating Enzyme E1, clone 2G2.3
Replaces:
05-624
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody specifically binds to ubiquitin activation enzyme E1. The antibody does not inhibit the enzymatic activity of E1.
Immunogen:
Ubiquitin activating enzyme E1.
Clone:
2G2.3
Isotype:
IgG1
Background Information:
The proteosome has long been thought of as the default mechanism for waste elimination within the cell. Recently, however, the proteosome has been recognized as an essential organelle necessary for the processing of antigens, the transcription factor NFkB and other proteins as well. Central to the function of the proteosome is the ubiquitinization of target proteins by a number of unique ubiquitin activation enzymes.
Species Reactivity:
- Human
- Mouse
Application Notes:
Western blot: 2-4 μg/mL
Immunohistochemistry
Immunoprecipitation
Sero-depletion
Optimal working dilutions must be determined by end user.
Immunohistochemistry
Immunoprecipitation
Sero-depletion
Optimal working dilutions must be determined by end user.
Presentation:
Liquid. Buffer = 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- UBA1
- UBE1X
- MGC4781
- A1S9T
- UBA1A
- A1S9
- UBE1
- A1ST
- GXP1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Key Applications:
- Immunoprecipitation
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P22314 # Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
SIZE: 1058 amino acids; 117849 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family.
MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
SIZE: 1058 amino acids; 117849 Da
SUBUNIT: Monomer (By similarity).
SIMILARITY: SwissProt: P22314 ## Belongs to the ubiquitin-activating E1 family.
MISCELLANEOUS: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Ubiquitin Activating Enzyme E1, clone 2G2.3
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody