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Mouse anti-GFAP (Catalog Number MAB360). Immunolocalization of GFAP (red) and MAP2 (blue) in rat hippocampus. Photo courtesy of Karl A. Kasischke & Pa...
Rabbit anti-MAP2 (Catalogue No. AB5622). Immunolocalization of MAP2 (green) and GFAP (red) in rat hippocampus. The slide was mounted with Vectashield ...
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Anti-Glial Fibrillary Acidic Protein, clone GA5
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| B, Ch, H, M, Po, R, Rb | IC, IH, IH(P), WB | Mouse | Ascites | Monoclonal Antibody |
UniProt序列号:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... 更多信息 »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... 更多信息 »
GenBank序列号:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein, clone GA5
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Alternate Names:
GFAP
Clone:
GA5
Specificity:
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Pig
- Rat
- Rabbit
Species Reactivity Note:
Reacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined.
Isotype:
IgG1
Immunogen:
Purified GFAP from porcine spinal cord.
Molecular Weight:
~ 51 kDa
Control:
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Presentation:
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- GFAP
- FLJ45472
Quality Assurance:
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Asc
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Format:
Ascites
Host:
Mouse
Purification Method:
Unpurified