Ordering Information
- : MAB368
- : 100 µL
- : Variable
Product Images
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Synaptophysin (1:500...
Anti-Synaptophysin Antibody | MAB368
Recommended Replacement for: MAB332| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H, M, Po, R | ELISA, IF, IH(P), WB | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Synaptophysin Antibody | MAB368
Replaces:
MAB332
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Localizes the 38 kD band of synaptophysin by immunoblotting. The monoclonal stains synaptophysin in neurons, neuromuscular junctions, paraganglia cells, pancreatic islet cells, anterior lymphomas and adrenal cells. Also detects synaptophysin in many types of benign and malignant neural and epithelial neuroendocrine neoplasmas.
Molecular Weight:
40 kDa
Immunogen:
Rat retina synaptophysin
Clone:
SVP-38
Isotype:
IgG1
Background Information:
Synaptophysin is a protein of the presynaptic vesicle exocytosis machinery interacting at the vesicle with VAMP/synaptobrevin. Synaptophysin structure consists of a hexameric protein of four transmembrane spans with cytoplasmic amino and carboxyl termini. Although its actual function remains elusive, synaptophysin remains a specific presynaptic marker for all neurons, neuroendocrine cells and neuroderived neoplasms.
Species Reactivity:
- Guinea Pig
- Human
- Mouse
- Pig
- Rat
Application Notes:
Immunohistochemistry/Immunofluorescence:
Immunoperoxidase/fluorescent labeling of formalin-fixed paraffin embedded or frozen tissue sections. Indirect immunoperoxidase at 1:100-1:200 on formalin-fixed, paraffin embedded rat cerebellum.
Immunoblotting:
1:1000, 38kDa.
ELISA:
Previous lot used ELISA.
Optimal working dilutions must be determined by the end user.
Immunoperoxidase/fluorescent labeling of formalin-fixed paraffin embedded or frozen tissue sections. Indirect immunoperoxidase at 1:100-1:200 on formalin-fixed, paraffin embedded rat cerebellum.
Immunoblotting:
1:1000, 38kDa.
ELISA:
Previous lot used ELISA.
Optimal working dilutions must be determined by the end user.
Control:
Brain tissue
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot:
1:500 dilution of this lot detected Synaptophysin on 10 μg of Mouse Brain lysates.
Western Blot:
1:500 dilution of this lot detected Synaptophysin on 10 μg of Mouse Brain lysates.
Purification Method:
Unpurified
Presentation:
Unpurified mouse monoclonal IgG1 liquid in buffer containing 0.1% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
SYP
Alternate Names:
- synaptophysin
- Synaptophysin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunofluorescence
- Immunohistochemistry (Paraffin)
- Western Blotting
Entrez Gene Summary:
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P08247 # Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
SIZE: 313 amino acids; 33845 Da
SUBUNIT: Homohexamer or homotetramer.
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
DOMAIN: SwissProt: P08247 The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation (By similarity).
SIMILARITY: Belongs to the synaptophysin/synaptobrevin family. & Contains 1 MARVEL domain.
Brand Family:
Chemicon
Product Name:
Anti-Synaptophysin Antibody | MAB368
Concentration:
Variable
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µL
Format:
Ascites
Host:
Mouse