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Ordering Information
- : MAB3684
- : 100 µL
Anti-Cyclin B1, clone V152
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH(P) | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Cyclin B1, clone V152
Trade Name:
Chemicon (Millipore)
Specificity:
Cyclin B. Cyclin B1 is a marker of cell proliferation and a key component of the cell progression machinery. MAB3684 works well for detecting Cyclin B1 by Western blot and immunohistochemistry.
Immunogen:
Hamster Cyclin B1.
Clone:
V152
Isotype:
IgG1
Species Reactivity:
- Human
- Mouse
Application Notes:
Western blot
Immunohistochemistry (works on paraffin embedded tissue sections)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry (works on paraffin embedded tissue sections)
Optimal working dilutions must be determined by the end user.
Presentation:
Ascites fluid. Liquid. Contains no preservative.
Storage Conditions:
Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CCNB1
- CCNB
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.
SIZE: 433 amino acids; 48337 Da
SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
SIZE: 433 amino acids; 48337 Da
SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Ascites
Host:
Mouse
Product Name:
Anti-Cyclin B1, clone V152
Antibody Type:
Monoclonal Antibody