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Ordering Information

  • : MAB374
  • : 200 μg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Product Family Information

Product Images

Immunocytochemistry Analysis:
Representative images from a previous lot.
Confolcal fluorescent analysis of HeLa and NIH/3T3 cells using MAB3...

Western Blot Analysis:
Representative lot data.
A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with an...

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Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5

Species Reactivity Key Applications Host Format Antibody Type
Ca, H, M, R, Rb, F, Fe, Po, not E. coli ELISA, IP, ICC, IF, IHC, WB Mouse Purified Monoclonal Antibody
Description:
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others.
Molecular Weight:
38 kDa
Immunogen:
Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle.
Clone:
Isotype:
Background Information:
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
Species Reactivity:
  • Canine
  • Human
  • Mouse
  • Rat
  • Rabbit
  • Fish
  • Feline
  • Pig
Species Reactivity Note:
Human, porcine, canine, rat, mouse, rabbit, cat, and fish skeletal muscle.
It has been reported that this antibody does not react with GAPDH from E. coli.
Non-Reactive Species:
E. coli
Application Notes:
ELISA:
1:1,000,000 dilution of a previous lot ws used for Immunoaffinity purification of GAPDH.

Immunocytochemistry:
1:100 to 1:300 dilution of a previous lot was used in immunocytochemistry in PBS-BSA 10 mg/mL. 4% PFA, with 0.02% PBS-Triton X-100; 3 min RT (Griffoni, 2001).

Western blot:
1:100 to 1:300. Recognizes a 36kDa band of the reduced monomer. Non-reduced GAPDH runs as a 146kDa tetramer.

Immunoaffinity purification of GAPDH.

Optimal working dilutions must be determined by end user.
Control:
It is expressed in all cells.
Quality Assurance:
Evaluated by Western Blot on A431 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected GLYCERALDEHYDE-3-PDH on 10 μg of A431 lysates.
Purification Method:
Protein A purfied
Presentation:
Purified Mouse monoclonal IgG1 in PBS, pH 7.4 with 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8ºC in undiluted aliquots for up to 6 months after date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
Alternate Names:
  • GAPDH
  • G3PDH
  • GAPD
  • OK/SW-cl.12
  • MGC88685
  • G3PD
  • EC 1.2.1.12
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified.
UniProt Summary:
FUNCTION: SwissProt: P04406 # Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway.
SIZE: 335 amino acids; 36053 Da
SUBUNIT: Homotetramer. Binds PRKCI.
SUBCELLULAR LOCATION: Cytoplasm. Note=Postnuclear and Perinuclear regions. Membrane.
SIMILARITY: SwissProt: P04406 ## Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Brand Family:
Chemicon
Product Name:
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
200 μg
Format:
Purified
Host:
Mouse
Applications:
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody, clone 6C5 detects level of Glyceraldehyde-3-Phosphate Dehydrogenase & has been published & validated for use in ELISA, IP, IC, IF, IH & WB.

Product Resources

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