Anti-NeuN, clone A60, Alexa Fluor®488 conjugated

Anti-NeuN, clone A60, Alexa Fluor®488 conjugated

NeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).

Neuron-Specific Nuclear Protein

A60

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells.

Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.

The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.

Immunohistochemistry

Immunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue.



Optimal working dilutions must be determined by end user.

• Human
• Mouse
• Rat

IgG1

Purified cell nuclei from mouse brain.

Brain tissue, most neuronal cell types throughout the adult nervous system

Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Maintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

• NeuN
• A60

Neuroscience

Neuronal & Glial Markers

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1 mg/mL

AlexaFluor®488

Chemicon (Millipore)

Protein A Purified

AlexaFluor®488

Monoclonal Antibody

Mouse